Molecular typing of a BHV-4 (bovine herpesvirus 4) field isolate

A new BHV-4 (bovine herpesvirus 4) isolated from a case of bovine interdigital dermatitis was characterized by PCR and restriction enzyme analysis. To determine whether the new isolate (PR/1) belonged to BHV-4, DNA from infected cells was specifically amplified by PCR. We used a set of primers spann...

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Bibliographic Details
Published in:Veterinary research communications 2000-09, Vol.24 (6), p.411-422
Main Authors: Donofrio, G, Cavirani, S, Flammini, C F, Scatozza, F
Format: Article
Language:English
Subjects:
Animals
ATK gene
Blotting, Southern - veterinary
Bovine herpesvirus 4
BVRF1 gene
BXRF1 gene
Cattle
Cattle Diseases - virology
Cytopathogenic Effect, Viral
Deoxyribonuclease HindIII - chemistry
Dermatitis - veterinary
Dermatitis - virology
DNA Primers - chemistry
DNA Probes
DNA, Viral - chemistry
DNA, Viral - isolation & purification
Electrophoresis, Agar Gel - veterinary
glycoprotein H gene
Herpesviridae - chemistry
Herpesviridae - classification
Herpesviridae - genetics
Herpesviridae Infections - veterinary
Herpesviridae Infections - virology
Italy
Polymerase Chain Reaction - veterinary
Restriction Mapping - veterinary
Sequence Analysis, DNA - veterinary
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Summary:A new BHV-4 (bovine herpesvirus 4) isolated from a case of bovine interdigital dermatitis was characterized by PCR and restriction enzyme analysis. To determine whether the new isolate (PR/1) belonged to BHV-4, DNA from infected cells was specifically amplified by PCR. We used a set of primers spanning a large 2.571 kb conserved region of the BHV-4 genome, including the 3' end of ORF1 (homologous to the EBV BVRF1 gene), ORF2 (homologous to the EBV BXRF1 gene), ORF3 (TK gene) and ORF4 (gH gene) 5' end, respectively. The identity of the amplified product was confirmed by HindIII restriction enzyme digestion and Southern hybridization. No product was observed from the DNA of other bovine herpesviruses tested. The restriction patterns of the PR/ 1 genome compared to DN 599, MOVAR 33/63 and LVR BHV-4 reference strains showed two kinds of differences, either related or not related to the prDNA (polyrepetitive DNA). Taken together, these data show that PR/ 1 is a new BHV-4. We would consider that the present report provides a scheme of work for diagnosis and typing of BHV-4 isolates.
ISSN:0165-7380
1573-7446
DOI:10.1023/A:1006478317972