In vivo and in vitro characteristics of double units of RBCs collected by apheresis with a single in-line WBC-reduction filter

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2001-11, Vol.41 (11), p.1373-1377
• Main Authors: Bandarenko, Nicholas, Rose, Michael, Kowalsky, Richard J., Baston, Robert K., Brecher, Mark E., Elfath, Mahoud D., Whitley, Pam, Heminway, Meritt, Holme, Stein
• Format: Article
• Language: English
• Subjects: 2-RBCF = 2 units of RBCs filtered (by new double-unit method); Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Blood Component Removal - instrumentation; Blood Component Removal - standards; Blood Donors; Blood Preservation; Blood Transfusion; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; Erythrocyte Count; Erythrocytes - cytology; Erythrocytes - physiology; Hematology; Humans; Investigative techniques, diagnostic techniques (general aspects); Leukapheresis - instrumentation; Leukocyte Count; Medical sciences; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Refrigeration; RT = room temperature; Time Factors; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1046/j.1537-2995.2001.41111373.x

BACKGROUND: A novel apheresis procedure for a blood separator (MCS+, Haemonetics) enables the collection of 2 WBC‐reduced RBC units in a single donation by using one disposable set with one in‐line WBC‐reduction filter (RC2H, Pall Corp.). The objective of this study was to evaluate the filtration performance in connection with different prefiltration RBC storage conditions and with the in vitro and in vivo storage quality of the filtered units. STUDY DESIGN AND METHODS: Sixty‐six 2‐unit RBC collection and gravity‐filtration procedures were completed at three sites, resulting in 132 RBC units. Filtration of the double RBC units was performed at room temperature (RT) within 8 hours of collection (n = 36) and under refrigeration (1‐6°C) for up to 24 hours (n = 10) and 72 hours (n = 20) before filtration. RBC quality was compared to that of nonfiltered apheresis RBC units (n = 10). RESULTS : Median filtration time was 6.5 and 14 minutes for units stored at RT and under refrigeration, respectively. All 132 RBC units had residual WBC counts