The egr‐1 promoter contains information for constitutive and inducible expression in transgenic mice

ABSTRACT Egr‐1 is an immediate early gene that couples short‐term changes in the extracellular milieu to long‐term changes in gene expression. Under in vitro conditions, the Egr‐1 gene is expressed in many cell types and is induced by a wide variety of extracellular signals. The mechanisms by which...

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Bibliographic Details
Published in:The FASEB journal 2000-10, Vol.14 (13), p.1870-1872
Main Authors: Tsai, Jo C., Liu, Lixin, Cooley, Brian C., DiChiara, Maria R., Topper, James N., Aird, William C.
Format: Article
Language:English
Subjects:
Animals
DNA-Binding Proteins - genetics
Early Growth Response Protein 1
endothelial cells
Gene Expression Regulation
Genes, Reporter
Hepatectomy
Immediate-Early Proteins - genetics
Lac Operon
Mice
Mice, Transgenic
Promoter Regions, Genetic
Tissue Distribution
Transcription Factors - genetics
transcriptional regulation
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Summary:ABSTRACT Egr‐1 is an immediate early gene that couples short‐term changes in the extracellular milieu to long‐term changes in gene expression. Under in vitro conditions, the Egr‐1 gene is expressed in many cell types and is induced by a wide variety of extracellular signals. The mechanisms by which the Egr‐1 gene is regulated in vivo remain poorly understood. In this study, we have generated transgenic mice with a construct containing 1200 bp of the mouse Egr‐1 promoter coupled to nuclear localized LacZ. In multiple independent lines of mice, reporter gene expression was detected in subsets of endothelial cells, vascular smooth‐muscle cells, cardiomyocytes, neurons, and hepatocytes. This pattern closely resembled that of the endogenous gene. After partial hepatectomy, reporter gene activity was upregulated between two‐ and fivefold in regenerating livers. Taken together, these findings suggest that the Egr‐1 promoter contains information for appropriate spatial and temporal expression in vivo.
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.99-1072fje