Glycyrrhizin and some analogues induce growth of primary cultured adult rat hepatocytes via epidermal growth factor receptors

• Published in: European journal of pharmacology 2001-11, Vol.431 (2), p.151-161
• Main Authors: Kimura, Mitsutoshi, Inoue, Hideo, Hirabayashi, Kazuhiro, Natsume, Hideshi, Ogihara, Masahiko
• Format: Article
• Language: English
• Subjects: Adenylyl Cyclase Inhibitors; Adult; Animals; Antibodies, Monoclonal - immunology; Biological and medical sciences; Cell Count; Cell Division - drug effects; Cells, Cultured; Cyclic AMP - metabolism; Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors; DNA - biosynthesis; DNA synthesis; Dose-Response Relationship, Drug; ErbB Receptors - agonists; ErbB Receptors - immunology; ErbB Receptors - metabolism; General pharmacology; Glycyrrhizic Acid - analogs & derivatives; Glycyrrhizic Acid - chemistry; Glycyrrhizic Acid - pharmacology; Glycyrrhizin; Hepatocyte; Hepatocytes - drug effects; Liver Regeneration; Male; Medical sciences; Mitogen-Activated Protein Kinase 1 - metabolism; Molecular Structure; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phosphorylation; Primary culture; Proliferation; rat; Rats; Rats, Wistar; Signal Transduction - drug effects; Time Factors
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/S0014-2999(01)01424-8

We investigated the effects of glycyrrhizin (GL-1) and some analogues on DNA synthesis and proliferation in serum-free primary cultures of adult rat hepatocytes. The hepatocytes underwent DNA synthesis and proliferation in response to GL-1 and some analogues. The effects of these agents occurred in a time- and dose-dependent manner. The proliferative potency as judged by half-maximal effective concentrations was in the following order: 18-β- H-glycyrrhetinic acid (GL-3; 4.5×10 −9 M)