BMP signaling regulates Nkx2-5 activity during cardiomyogenesis

Nkx2-5 regulates the transcription of muscle-specific genes during cardiomyogenesis. Nkx2-5 expression can induce cardiomyogenesis in aggregated P19 cells but not in monolayer cultures. In order to investigate the mechanism by which cellular aggregation regulates Nkx2-5 function, we examined the rol...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2001-11, Vol.509 (1), p.126-130
Main Authors: Jamali, Mina, Karamboulas, Christina, Rogerson, Parker J, Skerjanc, Ilona S
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
BMPs, bone morphogenetic proteins
Bone morphogenetic protein
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins - antagonists & inhibitors
Bone Morphogenetic Proteins - metabolism
Bone Morphogenetic Proteins - physiology
Cardiomyogenesis
Carrier Proteins
Cell Aggregation
Cell Differentiation
Cell Line
Cells, Cultured
DNA, Complementary - metabolism
Dose-Response Relationship, Drug
Embryonal carcinoma
Heart - embryology
Homeobox Protein Nkx-2.5
Homeodomain Proteins - metabolism
Mice
Microscopy, Fluorescence
MyHC, myosin heavy chain
Myocardium - metabolism
Nkx2-5
P19 cell
PGK, phosphoglycerate kinase
Plasmids - metabolism
Protein Biosynthesis
Proteins
Signal Transduction
Time Factors
Transcription Factors
Tumor Cells, Cultured
Xenopus Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nkx2-5 regulates the transcription of muscle-specific genes during cardiomyogenesis. Nkx2-5 expression can induce cardiomyogenesis in aggregated P19 cells but not in monolayer cultures. In order to investigate the mechanism by which cellular aggregation regulates Nkx2-5 function, we examined the role of bone morphogenetic protein 4 (BMP4). We showed that the expression of the BMP inhibitor, noggin, was sufficient to inhibit the induction of cardiomyogenesis by Nkx2-5 during cellular aggregation. Furthermore, soluble BMP4 could activate Nkx2-5 function in monolayer cultures, resulting in the formation of cardiomyocytes. Therefore, BMP signaling is necessary and sufficient for the regulation of Nkx2-5 activity during cardiomyogenesis in P19 cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)03151-9