Near-patient test for nicotine and its metabolites in saliva to assess smoking habit

Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried...

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Bibliographic Details
Published in:Annals of clinical biochemistry 2000-09, Vol.37 (5), p.666-673
Main Authors: COPE, G, NAYYAR, P, HOLDER, R, BROCK, G, CHAPPLE, I
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Colorimetry
Cotinine - analysis
Ent. Stomatology
Female
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Nicotine - chemistry
Nicotine - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Point-of-Care Systems - economics
Radioimmunoassay
Saliva - chemistry
Sensitivity and Specificity
Smoking
Smoking Cessation - methods
Statistics as Topic
Tobacco, tobacco smoking
Toxicology
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Summary:Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure nicotine and its metabolites by a colorimetric assay. The device can be used to give a semi-quantitative assessment of tobacco consumption by observing a colour change and comparing it to a reference chart. Alternatively, the test can be quantified by measuring the light absorbance with a colorimeter and determining the concentration of nicotine metabolites by reference to a cotinine standard. This method correlates with reported daily cigarette consumption (r=0·62, P< 0·0001) and nicotine yield of cigarettes smoked (r=0·35, P
ISSN:0004-5632
1758-1001
DOI:10.1258/0004563001899717