Direct transformation and plant regeneration of the haploid liverwort Marchantia polymorpha L

Thalli of the haploid liverwort Marchantial polymorpha were successfully used for direct particle bombardment with plasmid pMT, which carries a hygromycin phosphotransferase gene (hpt) controlled by the CaMV 35S promoter and the NOS polyadenylation region. Hygromycin-resistant cell masses arose from...

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Bibliographic Details
Published in:Transgenic research 2000-06, Vol.9 (3), p.179-185
Main Authors: TAKENAKA, Mizuki, YAMAOKA, Shohei, HANAJIRI, Tsutomu, SHIMIZU-UEDA, Yuu, YAMATO, Katsuyuki T, FUKUZAWA, Hideya, OHYAMA, Kanji
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Biological and medical sciences
Biotechnology
Blotting, Southern
Cells, Cultured - cytology
Cinnamates
DNA, Plant - analysis
Drug Resistance - genetics
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genome, Plant
hpt gene
hygromycin
Hygromycin B - analogs & derivatives
Hygromycin B - pharmacology
hygromycin phosphotransferase
Marchantia polymorpha
Methods. Procedures. Technologies
Phosphotransferases (Alcohol Group Acceptor) - biosynthesis
Phosphotransferases (Alcohol Group Acceptor) - genetics
Plants - enzymology
Plants - genetics
Plants, Genetically Modified
Plasmids - genetics
Transformation, Genetic
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Thalli of the haploid liverwort Marchantial polymorpha were successfully used for direct particle bombardment with plasmid pMT, which carries a hygromycin phosphotransferase gene (hpt) controlled by the CaMV 35S promoter and the NOS polyadenylation region. Hygromycin-resistant cell masses arose from the thallus surface and developed directly into hygromycin-resistant thalli. Southern blot analyses indicated that these thalli carried at least 1-4 copies of the hpt gene, which were stably transmitted to their asexual thallus progenies via gemma propagation for three generations. This transformation and direct plant regeneration protocol is expected to be a valuable tool for the molecular analysis of this lower land plant.
ISSN:0962-8819
1573-9368
DOI:10.1023/a:1008963410465