Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication

• Published in: Gene 2000-09, Vol.255 (2), p.219-227
• Main Authors: Abe, Eiji, Kuwahara, Kazuhiko, Yoshida, Mikoto, Suzuki, Mikio, Terasaki, Hidenori, Matsuo, Yoshinobu, Takahashi, Ei-ichi, Sakaguchi, Nobuo
• Format: Article
• Language: English
• Subjects: Acetyltransferases; Alternative splicing; Amino Acid Sequence; B-Lymphocytes - cytology; B-Lymphocytes - metabolism; Base Sequence; Blotting, Northern; Cell cycle; Cell Cycle Proteins - metabolism; Chromosome 21; Chromosome Mapping; Chromosomes, Human, Pair 21 - genetics; Cloning, Molecular; DNA Replication; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA-Binding Proteins; Female; GANP protein; Gene Expression; Genes - genetics; Germinal Center - metabolism; germinal center-associated nuclear protein; Humans; Immunohistochemistry; In Situ Hybridization; In Situ Hybridization, Fluorescence; Intracellular Signaling Peptides and Proteins; Minichromosome Maintenance Complex Component 3; Molecular Sequence Data; Nuclear phosphoprotein; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Phosphoproteins - genetics; Phosphoproteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Tissue Distribution; Tumor Cells, Cultured
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(00)00336-X

A 210 kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene ( hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7 kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells.