Effects of synthetic bis-beta-chloroethylamine estrogen derivatives on proliferation of skin sarcoma cells

The effects of four new synthetic bis-beta-chloroethylamine-containing estrogens and known cytostatic agents chlorophenacyl and estradiol mustard were compared on monolayer cultures of transformed L-929 fibroblasts (from murine skin sarcoma). The drugs within the concentration range of 10(-5)-5 10(-...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2000-06, Vol.129 (6), p.592-594
Main Authors: Semeikin, A V, Rzheznikov, V M, Mayatskaya, E E, Smirnova, Z S
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Hormonal - chemistry
Antineoplastic Agents, Hormonal - pharmacology
Cell Division - drug effects
Dose-Response Relationship, Drug
Estradiol Congeners - chemistry
Estradiol Congeners - pharmacology
Ethylamines - pharmacology
Fibroblasts - drug effects
Mice
Molecular Structure
Nitrogen Mustard Compounds - pharmacology
Sarcoma
Skin Neoplasms
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effects of four new synthetic bis-beta-chloroethylamine-containing estrogens and known cytostatic agents chlorophenacyl and estradiol mustard were compared on monolayer cultures of transformed L-929 fibroblasts (from murine skin sarcoma). The drugs within the concentration range of 10(-5)-5 10(-7)M inhibited proliferation of cultured cells by 67%. Chlorophenacyl displayed the least antiproliferative activity (15% inhibition at 10(-5) M). Steroid nucleus introduced into the molecule enhanced antiproliferative activity of test drug in comparison with chlorophenacyl, probably due to accumulation of the hormone-cytostatic molecules in cells. Estradiol had no effect on proliferative activity of L-929 cells, and no specific estrogen-binding sites were found in cultured transformed fibroblasts. The antiproliferative effect of hormone-cytostatics on this culture is not mediated via specific interactions with estrogen receptors.
ISSN:0007-4888
1573-8221
DOI:10.1007/BF02434886