Induction of lymphocyte recruitment in the absence of a detectable immune response

Lymphocyte recruitment from blood into the lymph node is thought to be initiated by the presence of antigen. In this study, we have used lymphatic cannulation in sheep to demonstrate that the adjuvant ISCOMATRIX™ can induce dramatic lymph node activation in the absence of antigen. Consistent pattern...

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Bibliographic Details
Published in:Vaccine 2000-10, Vol.19 (4), p.572-578
Main Authors: Windon, Ross G, Chaplin, Paul J, Beezum, Lorraine, Coulter, Alan, Cahill, Ross, Kimpton, Wayne, Drane, Debbie, Pearse, Martin, Sjölander, Anders, Tennent, Jan M, Scheerlinck, Jean-Pierre Y
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - administration & dosage
Adjuvants, Immunologic - chemistry
Animals
Antigen-Presenting Cells - immunology
Antigen-Presenting Cells - physiology
Applied microbiology
Biological and medical sciences
Cell Movement - immunology
Cytokine
Cytokines - biosynthesis
Erythrocytes - immunology
Female
Fundamental and applied biological sciences. Psychology
ISCOMATRIX
Lymph - cytology
Lymph - immunology
Lymphatic cannulation
Lymphocyte Activation
Lymphocytes - immunology
Lymphocytes - physiology
Microbiology
Neutrophils - immunology
Saponins - administration & dosage
Saponins - chemistry
Saponins - immunology
Sheep
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Lymphocyte recruitment from blood into the lymph node is thought to be initiated by the presence of antigen. In this study, we have used lymphatic cannulation in sheep to demonstrate that the adjuvant ISCOMATRIX™ can induce dramatic lymph node activation in the absence of antigen. Consistent patterns of node shutdown (decreased output) and cell recruitment (increased output) with minimal blast cell responses were observed indicating that an antigen-specific immune response is not required. Production of IL-6, IL-8 and IFN- γ, and the transient presence of red blood cells and neutrophils in the efferent lymph were associated with changes in efferent lymph cell trafficking. These early events may facilitate the screening of low frequency antigen-specific cells for binding to antigen and the subsequent amplification of the immune response.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(00)00148-1