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A single dose sub-unit vaccine protects against pneumonic plague
In this study, the protection afforded against aerosolised Yersinia pestis by injection of a single dose of an alhydrogel-adsorbed sub-unit vaccine has been compared with that given by an existing killed whole cell vaccine licensed for human use. The sub-unit vaccine, prepared by admixing F1 antigen...
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| Published in: | Vaccine 2000-10, Vol.19 (4), p.566-571 |
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| container_end_page | 571 |
| container_issue | 4 |
| container_start_page | 566 |
| container_title | Vaccine |
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| creator | Williamson, E.Diane Eley, Steven M. Stagg, Anthony J. Green, Michael Russell, Paul Titball, Richard W. |
| description | In this study, the protection afforded against aerosolised
Yersinia pestis by injection of a single dose of an alhydrogel-adsorbed sub-unit vaccine has been compared with that given by an existing killed whole cell vaccine licensed for human use. The sub-unit vaccine, prepared by admixing F1 antigen derived from a
Y. pestis cell culture supernatant with recombinant V antigen derived from an
E. coli cell lysate, fully protected an outbred strain of mouse against exposure to 10
6 CFU of virulent plague organisms (10
4 mouse lethal doses, MLD). In contrast, the whole cell vaccine provided only 16% protection against the same level of challenge. Furthermore, sub-unit vaccinees were able to clear the bacteria from their lungs post-challenge whereas bacteria were cultured from the lungs of a surviving KWC vaccinee post-challenge. In killed whole cell vaccinees, physiologically significant levels of IgG to F1 only were detectable and the levels of F1-specific IgG in serum and in broncho-alveolar washings were significantly lower (
p |
| doi_str_mv | 10.1016/S0264-410X(00)00159-6 |
| format | article |
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Yersinia pestis by injection of a single dose of an alhydrogel-adsorbed sub-unit vaccine has been compared with that given by an existing killed whole cell vaccine licensed for human use. The sub-unit vaccine, prepared by admixing F1 antigen derived from a
Y. pestis cell culture supernatant with recombinant V antigen derived from an
E. coli cell lysate, fully protected an outbred strain of mouse against exposure to 10
6 CFU of virulent plague organisms (10
4 mouse lethal doses, MLD). In contrast, the whole cell vaccine provided only 16% protection against the same level of challenge. Furthermore, sub-unit vaccinees were able to clear the bacteria from their lungs post-challenge whereas bacteria were cultured from the lungs of a surviving KWC vaccinee post-challenge. In killed whole cell vaccinees, physiologically significant levels of IgG to F1 only were detectable and the levels of F1-specific IgG in serum and in broncho-alveolar washings were significantly lower (
p<0.05) compared with sub-unit vaccinees. In sub-unit vaccinees, an IgG titre to the F1 and V antigens was detected in serum where it was significantly higher (
p<0.05) compared with broncho-alveolar washings suggesting that, at the time of challenge, protection is attributable mainly to the combined circulating IgG titre to the F1 and V sub-units. The enhanced protective efficacy of this sub-unit vaccine administered as a single dose compared with an existing vaccine has been demonstrated in an outbred animal model of pneumonic plague.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(00)00159-6</identifier><identifier>PMID: 11027822</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject><![CDATA[Animals ; Antibodies, Bacterial - biosynthesis ; Antibodies, Bacterial - blood ; Antigens, Bacterial - administration & dosage ; Bacterial Proteins - administration & dosage ; Bacteriology ; Biological and medical sciences ; Bronchoalveolar Lavage Fluid - immunology ; F1 antigen ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunization Schedule ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - blood ; Immunological correlates ; Lung - immunology ; Lung - microbiology ; Mice ; Microbiology ; Plague - immunology ; Plague - microbiology ; Plague - prevention & control ; Plague Vaccine - administration & dosage ; Pneumonic plague ; Pore Forming Cytotoxic Proteins ; Protection ; Single dose vaccine ; Spleen - immunology ; Sub-unit vaccine ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, Subunit - administration & dosage ; Yersinia pestis ; Yersinia pestis - immunology ; Yersinia pestis - isolation & purification ; Yersinia pestis - pathogenicity]]></subject><ispartof>Vaccine, 2000-10, Vol.19 (4), p.566-571</ispartof><rights>2000 Elsevier Science Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-d3c20edd38ad507422b328e78aac14f31cbc6e1f9d2d603b998f8c9a6ddfc6bb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=817275$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11027822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Williamson, E.Diane</creatorcontrib><creatorcontrib>Eley, Steven M.</creatorcontrib><creatorcontrib>Stagg, Anthony J.</creatorcontrib><creatorcontrib>Green, Michael</creatorcontrib><creatorcontrib>Russell, Paul</creatorcontrib><creatorcontrib>Titball, Richard W.</creatorcontrib><title>A single dose sub-unit vaccine protects against pneumonic plague</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>In this study, the protection afforded against aerosolised
Yersinia pestis by injection of a single dose of an alhydrogel-adsorbed sub-unit vaccine has been compared with that given by an existing killed whole cell vaccine licensed for human use. The sub-unit vaccine, prepared by admixing F1 antigen derived from a
Y. pestis cell culture supernatant with recombinant V antigen derived from an
E. coli cell lysate, fully protected an outbred strain of mouse against exposure to 10
6 CFU of virulent plague organisms (10
4 mouse lethal doses, MLD). In contrast, the whole cell vaccine provided only 16% protection against the same level of challenge. Furthermore, sub-unit vaccinees were able to clear the bacteria from their lungs post-challenge whereas bacteria were cultured from the lungs of a surviving KWC vaccinee post-challenge. In killed whole cell vaccinees, physiologically significant levels of IgG to F1 only were detectable and the levels of F1-specific IgG in serum and in broncho-alveolar washings were significantly lower (
p<0.05) compared with sub-unit vaccinees. In sub-unit vaccinees, an IgG titre to the F1 and V antigens was detected in serum where it was significantly higher (
p<0.05) compared with broncho-alveolar washings suggesting that, at the time of challenge, protection is attributable mainly to the combined circulating IgG titre to the F1 and V sub-units. The enhanced protective efficacy of this sub-unit vaccine administered as a single dose compared with an existing vaccine has been demonstrated in an outbred animal model of pneumonic plague.</description><subject>Animals</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - administration & dosage</subject><subject>Bacterial Proteins - administration & dosage</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - immunology</subject><subject>F1 antigen</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunization Schedule</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin G - blood</subject><subject>Immunological correlates</subject><subject>Lung - immunology</subject><subject>Lung - microbiology</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Plague - immunology</subject><subject>Plague - microbiology</subject><subject>Plague - prevention & control</subject><subject>Plague Vaccine - administration & dosage</subject><subject>Pneumonic plague</subject><subject>Pore Forming Cytotoxic Proteins</subject><subject>Protection</subject><subject>Single dose vaccine</subject><subject>Spleen - immunology</subject><subject>Sub-unit vaccine</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, Subunit - administration & dosage</subject><subject>Yersinia pestis</subject><subject>Yersinia pestis - immunology</subject><subject>Yersinia pestis - isolation & purification</subject><subject>Yersinia pestis - pathogenicity</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqF0E1LxDAQgOEgiq4fP0EpCKKH6iRp0_Ski_gFggcVvIV0Ml0i3XRtWsF_b9dd9Ogpl2cmw8vYIYdzDlxdPINQWZpxeDsFOAPgeZmqDTbhupCpyLneZJNfssN2Y3wHgFzycpvtcA6i0EJM2NU0iT7MGkpcGymJQ5UOwffJp0X0gZJF1_aEfUzszPoQ-2QRaJi3wWOyaOxsoH22Vdsm0sH63WOvtzcv1_fp49Pdw_X0McVMQJ86iQLIOamty6HIhKik0FRoa5FnteRYoSJel044BbIqS11rLK1yrkZVVXKPnaz2jhd9DBR7M_cRqWlsoHaIphBSgpblv5AXSmZawAjzFcSujbGj2iw6P7fdl-Fglo3NT2OzDGgAzE9jo8a5o_UHQzUn9ze1jjqC4zWwEW1Tdzagj79O80IU-aguV4rGap-eOhPRU0ByvhuLG9f6fw75BicvmHY</recordid><startdate>20001015</startdate><enddate>20001015</enddate><creator>Williamson, E.Diane</creator><creator>Eley, Steven M.</creator><creator>Stagg, Anthony J.</creator><creator>Green, Michael</creator><creator>Russell, Paul</creator><creator>Titball, Richard W.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20001015</creationdate><title>A single dose sub-unit vaccine protects against pneumonic plague</title><author>Williamson, E.Diane ; Eley, Steven M. ; Stagg, Anthony J. ; Green, Michael ; Russell, Paul ; Titball, Richard W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-d3c20edd38ad507422b328e78aac14f31cbc6e1f9d2d603b998f8c9a6ddfc6bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - biosynthesis</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial - administration & dosage</topic><topic>Bacterial Proteins - administration & dosage</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - immunology</topic><topic>F1 antigen</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunization Schedule</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin G - blood</topic><topic>Immunological correlates</topic><topic>Lung - immunology</topic><topic>Lung - microbiology</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Plague - immunology</topic><topic>Plague - microbiology</topic><topic>Plague - prevention & control</topic><topic>Plague Vaccine - administration & dosage</topic><topic>Pneumonic plague</topic><topic>Pore Forming Cytotoxic Proteins</topic><topic>Protection</topic><topic>Single dose vaccine</topic><topic>Spleen - immunology</topic><topic>Sub-unit vaccine</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, Subunit - administration & dosage</topic><topic>Yersinia pestis</topic><topic>Yersinia pestis - immunology</topic><topic>Yersinia pestis - isolation & purification</topic><topic>Yersinia pestis - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Williamson, E.Diane</creatorcontrib><creatorcontrib>Eley, Steven M.</creatorcontrib><creatorcontrib>Stagg, Anthony J.</creatorcontrib><creatorcontrib>Green, Michael</creatorcontrib><creatorcontrib>Russell, Paul</creatorcontrib><creatorcontrib>Titball, Richard W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Williamson, E.Diane</au><au>Eley, Steven M.</au><au>Stagg, Anthony J.</au><au>Green, Michael</au><au>Russell, Paul</au><au>Titball, Richard W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A single dose sub-unit vaccine protects against pneumonic plague</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2000-10-15</date><risdate>2000</risdate><volume>19</volume><issue>4</issue><spage>566</spage><epage>571</epage><pages>566-571</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>In this study, the protection afforded against aerosolised
Yersinia pestis by injection of a single dose of an alhydrogel-adsorbed sub-unit vaccine has been compared with that given by an existing killed whole cell vaccine licensed for human use. The sub-unit vaccine, prepared by admixing F1 antigen derived from a
Y. pestis cell culture supernatant with recombinant V antigen derived from an
E. coli cell lysate, fully protected an outbred strain of mouse against exposure to 10
6 CFU of virulent plague organisms (10
4 mouse lethal doses, MLD). In contrast, the whole cell vaccine provided only 16% protection against the same level of challenge. Furthermore, sub-unit vaccinees were able to clear the bacteria from their lungs post-challenge whereas bacteria were cultured from the lungs of a surviving KWC vaccinee post-challenge. In killed whole cell vaccinees, physiologically significant levels of IgG to F1 only were detectable and the levels of F1-specific IgG in serum and in broncho-alveolar washings were significantly lower (
p<0.05) compared with sub-unit vaccinees. In sub-unit vaccinees, an IgG titre to the F1 and V antigens was detected in serum where it was significantly higher (
p<0.05) compared with broncho-alveolar washings suggesting that, at the time of challenge, protection is attributable mainly to the combined circulating IgG titre to the F1 and V sub-units. The enhanced protective efficacy of this sub-unit vaccine administered as a single dose compared with an existing vaccine has been demonstrated in an outbred animal model of pneumonic plague.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11027822</pmid><doi>10.1016/S0264-410X(00)00159-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2000-10, Vol.19 (4), p.566-571 |
| issn | 0264-410X 1873-2518 |
| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Antibodies, Bacterial - biosynthesis Antibodies, Bacterial - blood Antigens, Bacterial - administration & dosage Bacterial Proteins - administration & dosage Bacteriology Biological and medical sciences Bronchoalveolar Lavage Fluid - immunology F1 antigen Female Fundamental and applied biological sciences. Psychology Humans Immunization Schedule Immunoglobulin G - biosynthesis Immunoglobulin G - blood Immunological correlates Lung - immunology Lung - microbiology Mice Microbiology Plague - immunology Plague - microbiology Plague - prevention & control Plague Vaccine - administration & dosage Pneumonic plague Pore Forming Cytotoxic Proteins Protection Single dose vaccine Spleen - immunology Sub-unit vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Subunit - administration & dosage Yersinia pestis Yersinia pestis - immunology Yersinia pestis - isolation & purification Yersinia pestis - pathogenicity |
| title | A single dose sub-unit vaccine protects against pneumonic plague |
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