Pyrimidine base catabolism in Pseudomonas putida biotype B

Reductive catabolism of the pyrimidine bases uracil and thymine was found to occur in Pseudomonas putida biotype B. The pyrimidine reductive catabolic pathway enzymes dihydropyrimidine dehydrogenase, dihydropyrimidinase and N-carbamoyl-beta-alanine amidohydrolase activities were detected in this pse...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2001-10, Vol.80 (2), p.163-167
Main Author: WEST, Thomas P
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Biodegradation of pollutants
Biological and medical sciences
Biotechnology
Carbon - metabolism
Culture Media
Dehydrogenase
Environment and pollution
Enzymatic activity
Enzymes
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
Industrial applications and implications. Economical aspects
Microbiology
Motility, taxis
N-Carabomyl-b-alanine amidohydrolase
NAD - metabolism
NADH
NADP - metabolism
NADPH
Nitrogen
Oxidation-Reduction
Pseudomonas putida
Pseudomonas putida - classification
Pseudomonas putida - growth & development
Pseudomonas putida - metabolism
Succinates - metabolism
thymine
Thymine - metabolism
uracil
Uracil - metabolism
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Summary:Reductive catabolism of the pyrimidine bases uracil and thymine was found to occur in Pseudomonas putida biotype B. The pyrimidine reductive catabolic pathway enzymes dihydropyrimidine dehydrogenase, dihydropyrimidinase and N-carbamoyl-beta-alanine amidohydrolase activities were detected in this pseudomonad. The initial reductive pathway enzyme dihydropyrimidine dehydrogenase utilized NADH or NADPH as its nicotinamide cofactor. The source of nitrogen in the culture medium influenced the reductive pathway enzyme activities and, in particular, dihydropyrimidinase activity was highly affected by nitrogen source. The reductive pathway enzyme activities in succinate-grown P. putida biotype B cells were induced when uracil served as the nitrogen source.
ISSN:0003-6072
1572-9699
DOI:10.1023/A:1012275512136