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A Novel Spermatogenesis-Related Factor-1 Gene Expressed in Maturing Rat Testis

A rat gene with testis-specific expression coinciding with spermatogenesis was cloned by differential display. This spermatogenesis-related factor-1 (SRF-1) gene was not expressed in other organs. Testicular expression was detected from 5 weeks of age and increased up to 15 weeks; this level of expr...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-12, Vol.289 (4), p.888-893
Main Authors: Yamano, Yoshiaki, Ohyama, Kenji, Sano, Tomoaki, Ohta, Masanori, Shimada, Akinori, Hirakawa, Yuri, Sugimoto, Miyako, Morishima, Isao
Format: Article
Language:English
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Summary:A rat gene with testis-specific expression coinciding with spermatogenesis was cloned by differential display. This spermatogenesis-related factor-1 (SRF-1) gene was not expressed in other organs. Testicular expression was detected from 5 weeks of age and increased up to 15 weeks; this level of expression was maintained for 63 weeks. The 750-bp cloned gene was coded for an open reading frame of 202 amino acids. According to in situ hybridization at 7 weeks, this gene was expressed mainly in spermatocyte. The gene product may function as a molecular motor in meiosis, as the deduced amino acid sequence showed partial homology with kinesin-related proteins. The action of this gene and its product with respect to division of reproductive cells requires further investigation.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.6048