Rice Straw as a Support for Immobilization of Microbial Lipase

Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 22 factorial design. Higher immobilization yields (>70%)...

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Bibliographic Details
Published in:Biotechnology progress 2001, Vol.17 (6), p.1061-1064
Main Authors: de Castro, Heizir F., de Lima, Rosemar, Roberto, Inês C.
Format: Article
Language:English
Subjects:
1-Butanol - chemistry
Biological and medical sciences
Biotechnology
Butyrates - chemistry
Candida - enzymology
Derivatives
Enzymes, Immobilized - chemistry
Esters - chemistry
Fundamental and applied biological sciences. Psychology
Hydrolysis
Immobilization of enzymes and other molecules
Immobilization techniques
Indicators and Reagents
Lipase - chemistry
Methods. Procedures. Technologies
Molecular weight
Olive Oil
Oryza - chemistry
Plant Oils
Plant Stems - chemistry
Polyethylene Glycols
Straw
Synthesis (chemical)
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Summary:Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 22 factorial design. Higher immobilization yields (>70%) were attained when the lipase loading was 95 units/mg of dry support, independent of PEG molecular weight. All derivatives showed high hydrolytic and synthetic activities. This work provides preliminary results on the use of agricultural residues as a support matrix for immobilizing lipase and on the application of the resulting derivatives to butyl butyrate synthesis as a study model.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp010099t