Analysis of Biofilm Structure and Gene Expression Using Fluorescence Dual Labeling

The use of biofilms for the degradation of recalcitrant environmental contaminants or for the production of secondary metabolites necessitates understanding and controlling gene expression. In this work, dual labeling with green fluorescent protein (GFP) variants was used to investigate inducible ge...

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Bibliographic Details
Published in:Biotechnology progress 2001, Vol.17 (6), p.1180-1182
Main Authors: Gilbert, Eric S., Khlebnikov, Artem, Cowan, Stacie E., Keasling, Jay D.
Format: Article
Language:English
Subjects:
Biofilms
Biological and medical sciences
Biotechnology
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli - ultrastructure
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling - methods
Gene Expression Regulation, Bacterial - genetics
Green Fluorescent Proteins
Luminescent Proteins - biosynthesis
Luminescent Proteins - genetics
Methods. Procedures. Technologies
Others
Various methods and equipments
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Summary:The use of biofilms for the degradation of recalcitrant environmental contaminants or for the production of secondary metabolites necessitates understanding and controlling gene expression. In this work, dual labeling with green fluorescent protein (GFP) variants was used to investigate inducible gene expression in a biofilm. Colocalization of GFP emissions was used to determine regions of attached cells and to correlate structure and activity within the biofilm. The labeling strategy reported here is unique in that the two GFP signals were distinguished by differential excitation rather than differential emission.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp0101031