Optimization of fermentation reaction conditions for preparation of PATE enzyme by Erwinia carotovora IFO3830 by TFCCRD method

The fermentation conditions for preparation of polygalacturonic acid transeliminase (PATE) enzyme by Erwinia carotovora IFO3830 were optimized for seed ratio, vibration rate, and temperature by the TFCCRD method. The results indicated that the optimum fermentation conditions for E. carotovora IFO 38...

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Bibliographic Details
Published in:Biotechnology progress 2001-11, Vol.17 (6), p.1014-1019
Main Authors: FENGPING DING, NORITOMI, Hidetaka, NAGAHAMA, Kunio
Format: Article
Language:English
Subjects:
Algorithms
Bacteriological Techniques - standards
Biological and medical sciences
Biotechnology
Culture Media
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Pectobacterium carotovorum - enzymology
Polysaccharide-Lyases - biosynthesis
Production of selected enzymes
Spectrophotometry, Ultraviolet
Temperature
Vibration
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Summary:The fermentation conditions for preparation of polygalacturonic acid transeliminase (PATE) enzyme by Erwinia carotovora IFO3830 were optimized for seed ratio, vibration rate, and temperature by the TFCCRD method. The results indicated that the optimum fermentation conditions for E. carotovora IFO 3830 were that seed ratio, vibration rate, and temperature were 5% v/v, 113 min(-1), and 29 degrees C, respectively.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp0100934