Differential gene expression in neutrophils from patients with generalized aggressive periodontitis

Differential gene expression was investigated in neutrophils stimulated with N‐formyl‐methionyl‐leucyl‐phenylalanine using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP‐PCR). The cells were isolated from 3 groups of subjects: patients with generalized aggressive periodontit...

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Bibliographic Details
Published in:Journal of periodontal research 2001-12, Vol.36 (6), p.390-397
Main Authors: Kubota, Takehiko, Morozumi, Toshiya, Shimizu, Kunihiko, Sugita, Noriko, Kobayashi, Tetsuo, Yoshie, Hiromasa
Format: Article
Language:English
Subjects:
Adult
Aggressive Periodontitis - genetics
Aggressive Periodontitis - immunology
Aggressive Periodontitis - metabolism
Biological and medical sciences
Case-Control Studies
Cell Adhesion Molecules
Dentistry
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Dual-Specificity Phosphatases
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Female
Gene Expression
Heat Shock Transcription Factors
Humans
Intercellular Signaling Peptides and Proteins
Intracellular Signaling Peptides and Proteins
Kruppel-Like Factor 6
Kruppel-Like Transcription Factors
Male
Medical sciences
Mitogen-Activated Protein Kinase Phosphatases
neutrophil
Neutrophils - metabolism
Non tumoral diseases
Otorhinolaryngology. Stomatology
periodontitis
Periodontitis - genetics
Periodontitis - immunology
Periodontitis - metabolism
Phosphofructokinases - biosynthesis
Phosphofructokinases - genetics
Protein Biosynthesis
Protein Tyrosine Phosphatases - biosynthesis
Protein Tyrosine Phosphatases - genetics
Proteins - genetics
Proto-Oncogene Proteins
Random Amplified Polymorphic DNA Technique
RAP-PCR
Reverse Transcriptase Polymerase Chain Reaction
RNA - analysis
Sequence Analysis, DNA
Trans-Activators - biosynthesis
Trans-Activators - genetics
Transcription Factors - biosynthesis
Transcription Factors - genetics
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Summary:Differential gene expression was investigated in neutrophils stimulated with N‐formyl‐methionyl‐leucyl‐phenylalanine using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP‐PCR). The cells were isolated from 3 groups of subjects: patients with generalized aggressive periodontitis (Aggressive‐P, n=6), generalized chronic periodontitis (Chronic‐P, n=6) and healthy controls (H, n=8). Our results show that 37 genes were upregulated, while 27 genes were down‐regulated in all Aggressive‐P neutrophils by using RAP‐PCR with 45 primer pairs. Reverse transcription‐PCR analyses revealed that mRNA levels were significantly different (p
ISSN:0022-3484
1600-0765
DOI:10.1034/j.1600-0765.2001.360607.x