Transendothelial permeability of chlorpyrifos in RBE4 monolayers is modulated by astrocyte-conditioned medium

• Published in: Brain research. Molecular brain research. 2001-12, Vol.97 (1), p.43-50
• Main Authors: Yang, Jian, Mutkus, Lysette A, Sumner, Darrell, Stevens, James T, Eldridge, J.Charles, Strandhoy, Jack W, Aschner, Michael
• Format: Article
• Language: English
• Subjects: Animals; Astrocyte; Astrocytes - metabolism; ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism; Biological and medical sciences; Blood-Brain Barrier - drug effects; Blood–brain barrier; Brain - cytology; Cadherins - biosynthesis; Cadherins - genetics; Cadherins - physiology; Calcium Channel Blockers - pharmacology; Cell Line, Transformed - drug effects; Cell Line, Transformed - metabolism; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Chlorpyrifos - metabolism; Culture Media, Conditioned - pharmacology; Drug Resistance, Multiple; E-cadherin; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; In vitro; Permeability; Rats; Verapamil - pharmacology; Vertebrates: nervous system and sense organs
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/S0169-328X(01)00296-0

The immortalized rat brain endothelium 4 (RBE4) cell line preserves many features of the in vivo brain endothelium. It has been used as an in vitro model of the blood–brain barrier (BBB). Astrocyte–endothelial cell interactions are crucial for maintenance of BBB characteristics. The present study investigated morphological and permeability properties of the RBE4 cell line. Immunohistochemical studies showed positive staining in RBE4 cells for E-cadherin, a Ca 2+-dependent cell–cell adhesion molecule. Western blot immunoassay showed that RBE4 cells consistently express E-cadherin and that its expression significantly increased ( P