Lateral mobility and anchoring of recombinant GABAA receptors depend on subunit composition

• Published in: Cell motility and the cytoskeleton 2001-10, Vol.50 (2), p.89-100
• Main Authors: Peran, Macarena, Hicks, Barry W., Peterson, Nancy L., Hooper, Helen, Salas, Ramiro
• Format: Article
• Language: English
• Subjects: anchoring; Animals; Cell Line; Cell Membrane - physiology; Cell Membrane - ultrastructure; COS Cells; cytoskeleton; Cytoskeleton - physiology; Endoplasmic Reticulum - physiology; fluorescence photobleach recovery; gamma-Aminobutyric Acid - physiology; Humans; Neural Inhibition - physiology; Neurons - cytology; Neurons - physiology; PC12 Cells; Protein Subunits - genetics; Protein Transport - physiology; Rats; Receptor Aggregation - genetics; receptor mobility; Receptors, GABA-A - physiology; Recombinant Fusion Proteins - physiology; single particle tracking; Synaptic Transmission - physiology; Transfection
• ISSN: 0886-1544; 1097-0169
• DOI: 10.1002/cm.1043

The clustering of type A γ‐aminobutyric acid receptors (GABAAR) at discrete and functionally significant domains on the nerve cell surface is an important determinant in the integration of synaptic inputs. To discern the role that the subunits of the GABAAR play in determining the receptor's cell surface topography and mobility, the α1, β1, β3, and γ2s subunits were transfected into COS7, HEK293, and PC12 cells and the distribution and cell surface mobility of these recombinant receptors were examined. Our results show that α1 subunits are retained in the endoplasmic reticulum while β1 and β3 subunits are sorted to the plasma membrane where they form clusters. Co‐expression and co‐assembly of α1 and β3 subunits result in the rescue of intracellular α1 subunits, which are transported as αβ subunit complexes to the cell surface where they formed clusters. Fluorescence photobleach recovery and single particle tracking of recombinant receptors show that, despite clustering, β3 subunit homooligomers are mobile within a cell surface domain. Inclusion of α1 in β3 or β3γ2s complexes, however, dramatically reduces the receptor's lateral mobility in COS 7 and PC12 cells and anchors GABAARs on the cell surface, suggesting the formation of a direct link to a component of the cytoskeleton. The mobility of recombinant receptors that include the α1 subunit mirrors the mobility of GABAARs on cell bodies and dendrites of cortical and spinal cord neurons. The results suggest that incorporation of α1 subunits give rise to a population of GABAARs that are immobilized on the cell surface. Cell Motil. Cytoskeleton 50:89–100, 2001. © 2001 Wiley‐Liss, Inc.