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Comparative sequence analysis identified mutations outside the NSP4 cytotoxic domain of tissue culture-adapted ATCC-Wa strain of human rotavirus and a novel inter-species variable domain in its C-terminus
Complete nucleotide sequence of the tissue culture-adapted ATCC*-Wa strain of human rotavirus NSP4 was determined. Sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (A or G) in the coding sequence (NSP4-A or NSP4-G) leading to a change from neutr...
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Published in: | Archives of virology 2000-01, Vol.145 (9), p.1789-1799 |
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description | Complete nucleotide sequence of the tissue culture-adapted ATCC*-Wa strain of human rotavirus NSP4 was determined. Sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (A or G) in the coding sequence (NSP4-A or NSP4-G) leading to a change from neutral glutamine⁹⁷in NSP4-A to a positively charged arginine⁹⁷in NSP4-G originating from the same ATCC-Wa preparation. In addition to this, both forms of ATCC-Wa NSP4 revealed three mutations at nucleotide positions 88 (T to C), 142 (C to T) and 572 (G to A), when compared to the previously reported NSP4 sequence from virulent Wa strain. The former two mutations were in the coding sequence and resulted in a leucine¹⁶to serine¹⁶and a proline³⁴to leucine³⁴change, while the third mutation was in the 3′ non-coding region of the gene. The two amino acid changes may contribute to the ‘tissue culture-adaptation’ of ATCC-Wa strain. The ATCC-Wa NSP4 sequence was found to differ from the previously reported NSP4 sequence of attenuated Wa strain by lacking a mutation at 133 (T to C), though the mutations at 88 and 142 were present in both strains. Furthermore, comparison of deduced amino acid sequence of NSP4 from human, bovine, porcine and simian rotavirus strains identified a seven-residue (positions 135–141) inter-species variable domain in its C-terminal region. |
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V ; Atreya, C. D</creator><creatorcontrib>Krishna Mohan, K. V ; Atreya, C. D</creatorcontrib><description>Complete nucleotide sequence of the tissue culture-adapted ATCC*-Wa strain of human rotavirus NSP4 was determined. Sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (A or G) in the coding sequence (NSP4-A or NSP4-G) leading to a change from neutral glutamine⁹⁷in NSP4-A to a positively charged arginine⁹⁷in NSP4-G originating from the same ATCC-Wa preparation. In addition to this, both forms of ATCC-Wa NSP4 revealed three mutations at nucleotide positions 88 (T to C), 142 (C to T) and 572 (G to A), when compared to the previously reported NSP4 sequence from virulent Wa strain. The former two mutations were in the coding sequence and resulted in a leucine¹⁶to serine¹⁶and a proline³⁴to leucine³⁴change, while the third mutation was in the 3′ non-coding region of the gene. The two amino acid changes may contribute to the ‘tissue culture-adaptation’ of ATCC-Wa strain. The ATCC-Wa NSP4 sequence was found to differ from the previously reported NSP4 sequence of attenuated Wa strain by lacking a mutation at 133 (T to C), though the mutations at 88 and 142 were present in both strains. Furthermore, comparison of deduced amino acid sequence of NSP4 from human, bovine, porcine and simian rotavirus strains identified a seven-residue (positions 135–141) inter-species variable domain in its C-terminal region.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s007050070056</identifier><identifier>PMID: 11043941</identifier><language>eng</language><publisher>Wien: Springer-Verlag</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Amino Acid Substitution ; amino acids ; Animals ; Base Sequence ; Biological and medical sciences ; Cattle ; Cell Line ; Cloning, Molecular ; cytotoxicity ; DNA-Directed RNA Polymerases ; Fundamental and applied biological sciences. Psychology ; genes ; Genome, Viral ; Human rotavirus ; Humans ; Isoelectric Point ; Leucine ; Macaca ; Microbiology ; Molecular Sequence Data ; Mutation ; NS4 protein ; NSP4 protein ; nucleotide sequences ; Proline ; Rotavirus ; Rotavirus - chemistry ; Rotavirus - genetics ; sequence analysis ; Serine ; Swine ; Techniques used in virology ; Viral Nonstructural Proteins - chemistry ; Viral Nonstructural Proteins - genetics ; Virology</subject><ispartof>Archives of virology, 2000-01, Vol.145 (9), p.1789-1799</ispartof><rights>2001 INIST-CNRS</rights><rights>2000 Springer-Verlag/ Wien</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-60bebb4a9e69b7912eea5ca25681f919c068ef884159777129a8a9f700b26dbe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=997915$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11043941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krishna Mohan, K. V</creatorcontrib><creatorcontrib>Atreya, C. D</creatorcontrib><title>Comparative sequence analysis identified mutations outside the NSP4 cytotoxic domain of tissue culture-adapted ATCC-Wa strain of human rotavirus and a novel inter-species variable domain in its C-terminus</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>Complete nucleotide sequence of the tissue culture-adapted ATCC*-Wa strain of human rotavirus NSP4 was determined. Sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (A or G) in the coding sequence (NSP4-A or NSP4-G) leading to a change from neutral glutamine⁹⁷in NSP4-A to a positively charged arginine⁹⁷in NSP4-G originating from the same ATCC-Wa preparation. In addition to this, both forms of ATCC-Wa NSP4 revealed three mutations at nucleotide positions 88 (T to C), 142 (C to T) and 572 (G to A), when compared to the previously reported NSP4 sequence from virulent Wa strain. The former two mutations were in the coding sequence and resulted in a leucine¹⁶to serine¹⁶and a proline³⁴to leucine³⁴change, while the third mutation was in the 3′ non-coding region of the gene. The two amino acid changes may contribute to the ‘tissue culture-adaptation’ of ATCC-Wa strain. The ATCC-Wa NSP4 sequence was found to differ from the previously reported NSP4 sequence of attenuated Wa strain by lacking a mutation at 133 (T to C), though the mutations at 88 and 142 were present in both strains. Furthermore, comparison of deduced amino acid sequence of NSP4 from human, bovine, porcine and simian rotavirus strains identified a seven-residue (positions 135–141) inter-species variable domain in its C-terminal region.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Amino Acid Substitution</subject><subject>amino acids</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>cytotoxicity</subject><subject>DNA-Directed RNA Polymerases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genome, Viral</subject><subject>Human rotavirus</subject><subject>Humans</subject><subject>Isoelectric Point</subject><subject>Leucine</subject><subject>Macaca</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>NS4 protein</subject><subject>NSP4 protein</subject><subject>nucleotide sequences</subject><subject>Proline</subject><subject>Rotavirus</subject><subject>Rotavirus - chemistry</subject><subject>Rotavirus - genetics</subject><subject>sequence analysis</subject><subject>Serine</subject><subject>Swine</subject><subject>Techniques used in virology</subject><subject>Viral Nonstructural Proteins - chemistry</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqF0luL1TAQB_AiintcffRVAwviSzVJ00sel-INFhV2Fx_LNJ26WdqmZpKD5zv6oczhHK8PCiGB5Mf8YTJZ9ljwF4Lz-iWljZf7jZfVnWwjVCHzptbN3WzDC67ypuLNSfaA6JbzdFGU97MTIbgqtBKb7Fvr5hU8BLtFRvgl4mKQwQLTjiwxO-AS7GhxYHMMSbmFmIuB0gMLN8jeX35UzOyCC-6rNWxwM9iFuZEFSxSRmTiF6DGHAdaQqpxftW3-CRgFf4Q3cYaFeRdga32klD0wYIvb4sTsEtDntKKxSGwL3kI_4Y-U_QrE2jyh2S6RHmb3RpgIHx3P0-z69aur9m1-8eHNu_b8IjdK65BXvMe-V6Cx0n2thUSE0oAsq0aMWmjDqwbHplGi1HVdC6mhAT2mDveyGnosTrNnh7qrd6ljFLrZksFpggVdpK6WRSnKsv4vFHUlVQpJ8Pm_YaVkJWpVyUTP_qK3Lvr0YUlxqWpVFkIklR-U8Y7I49it3s7gdwl1-8Hp_hic5J8cq8Z-xuGXPk7Kb7FABqbRw2Is_XRap0aWST09qBFcB599EteXkouUw1MvZVF8BwJr1fI</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Krishna Mohan, K. 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V</au><au>Atreya, C. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative sequence analysis identified mutations outside the NSP4 cytotoxic domain of tissue culture-adapted ATCC-Wa strain of human rotavirus and a novel inter-species variable domain in its C-terminus</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>145</volume><issue>9</issue><spage>1789</spage><epage>1799</epage><pages>1789-1799</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Complete nucleotide sequence of the tissue culture-adapted ATCC*-Wa strain of human rotavirus NSP4 was determined. Sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (A or G) in the coding sequence (NSP4-A or NSP4-G) leading to a change from neutral glutamine⁹⁷in NSP4-A to a positively charged arginine⁹⁷in NSP4-G originating from the same ATCC-Wa preparation. In addition to this, both forms of ATCC-Wa NSP4 revealed three mutations at nucleotide positions 88 (T to C), 142 (C to T) and 572 (G to A), when compared to the previously reported NSP4 sequence from virulent Wa strain. The former two mutations were in the coding sequence and resulted in a leucine¹⁶to serine¹⁶and a proline³⁴to leucine³⁴change, while the third mutation was in the 3′ non-coding region of the gene. The two amino acid changes may contribute to the ‘tissue culture-adaptation’ of ATCC-Wa strain. The ATCC-Wa NSP4 sequence was found to differ from the previously reported NSP4 sequence of attenuated Wa strain by lacking a mutation at 133 (T to C), though the mutations at 88 and 142 were present in both strains. Furthermore, comparison of deduced amino acid sequence of NSP4 from human, bovine, porcine and simian rotavirus strains identified a seven-residue (positions 135–141) inter-species variable domain in its C-terminal region.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer-Verlag</pub><pmid>11043941</pmid><doi>10.1007/s007050070056</doi><tpages>11</tpages></addata></record> |
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subjects | Amino Acid Sequence amino acid sequences Amino Acid Substitution amino acids Animals Base Sequence Biological and medical sciences Cattle Cell Line Cloning, Molecular cytotoxicity DNA-Directed RNA Polymerases Fundamental and applied biological sciences. Psychology genes Genome, Viral Human rotavirus Humans Isoelectric Point Leucine Macaca Microbiology Molecular Sequence Data Mutation NS4 protein NSP4 protein nucleotide sequences Proline Rotavirus Rotavirus - chemistry Rotavirus - genetics sequence analysis Serine Swine Techniques used in virology Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - genetics Virology |
title | Comparative sequence analysis identified mutations outside the NSP4 cytotoxic domain of tissue culture-adapted ATCC-Wa strain of human rotavirus and a novel inter-species variable domain in its C-terminus |
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