Scc1/Rad21/Mcd1 Is Required for Sister Chromatid Cohesion and Kinetochore Function in Vertebrate Cells

Proteolytic cleavage of the cohesin subunit Scc1 is a consistent feature of anaphase onset, although temporal differences exist between eukaryotes in cohesin loss from chromosome arms, as distinct from centromeres. We describe the effects of genetic deletion of Scc1 in chicken DT40 cells. Scc1 loss...

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Bibliographic Details
Published in:Developmental cell 2001-12, Vol.1 (6), p.759-770
Main Authors: Sonoda, Eiichiro, Matsusaka, Takahiro, Morrison, Ciaran, Vagnarelli, Paola, Hoshi, Osamu, Ushiki, Tatsuo, Nojima, Kuniharu, Fukagawa, Tatsuo, Waizenegger, Irene C., Peters, Jan-Michael, Earnshaw, William C., Takeda, Shunichi
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Cell Cycle - physiology
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Line
Cell Nucleus - metabolism
CENP-C protein
CENP-H protein
Chickens
Chromatids - metabolism
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Cohesins
DNA Repair
Doxycycline - pharmacology
Flow Cytometry
Fungal Proteins
Humans
In Situ Hybridization, Fluorescence
Kinetochores - metabolism
Macromolecular Substances
Mcd1 protein
Microscopy, Atomic Force
Microscopy, Fluorescence
Nuclear Proteins - metabolism
Phenotype
Phosphoproteins
Protein Subunits
Rad21 protein
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Saccharomyces cerevisiae Proteins
Scc1 protein
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Summary:Proteolytic cleavage of the cohesin subunit Scc1 is a consistent feature of anaphase onset, although temporal differences exist between eukaryotes in cohesin loss from chromosome arms, as distinct from centromeres. We describe the effects of genetic deletion of Scc1 in chicken DT40 cells. Scc1 loss caused premature sister chromatid separation but did not disrupt chromosome condensation. Scc1 mutants showed defective repair of spontaneous and induced DNA damage. Scc1-deficient cells frequently failed to complete metaphase chromosome alignment and showed chromosome segregation defects, suggesting aberrant kinetochore function. Notably, the chromosome passenger INCENP did not localize normally to centromeres, while the constitutive kinetochore proteins CENP-C and CENP-H behaved normally. These results suggest a role for Scc1 in mitotic regulation, along with cohesion.
ISSN:1534-5807
1878-1551
DOI:10.1016/S1534-5807(01)00088-0