Development of a Simple Cell Invasion Assay System

Cell invasion assay is important for studying various biological events, such as inflammation, cancer metastasis, and angiogenesis. In this study, we developed a simple method for the quantification of cell invasion by using a culture insert with fluorescence blocking micropore membrane (FBM). Fluor...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2000/10/01, Vol.23(10), pp.1264-1266
Main Authors: YAMAKAWA, Satoru, FURUYAMA, Yukihiro, OKU, Naoto
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Assay
Cell differentiation, maturation, development, hematopoiesis
Cell Physiological Phenomena - drug effects
Cell physiology
Endothelial Growth Factors - pharmacology
Endothelium, Vascular - cytology
Fluorescent Dyes
fluoresence blocking membrane
Fundamental and applied biological sciences. Psychology
GFP
Green Fluorescent Proteins
Humans
HUVEC
invasion
Luminescent Proteins
Lymphokines - pharmacology
Molecular and cellular biology
Tumor Cells, Cultured
Umbilical Veins - cytology
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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Summary:Cell invasion assay is important for studying various biological events, such as inflammation, cancer metastasis, and angiogenesis. In this study, we developed a simple method for the quantification of cell invasion by using a culture insert with fluorescence blocking micropore membrane (FBM). Fluorescence labeled cells were simply added to a culture insert with a 8 μm-pore FBM precoated with Matrigel and incubated for an appropriate duration. Then, the FBM was examined under a fluorescence microscope to count the invaded cell number. By this method, accurate invasion assay is easily performed without the steps of fixation and staining of cells and removal of cells which do not invade.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.23.1264