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Cre-mediated transgene activation in the developing and adult mouse brain
The neuron‐specific rat enolase (NSE) promoter was employed to establish transgenic mice expressing Cre recombinase in the central nervous system. Founders were crossed with dormant lacZ indicator mice and specificity as well as efficiency of Cre‐mediated transgene activation was determined by PCR a...
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Published in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2001-11, Vol.31 (3), p.118-125 |
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creator | Cinato, Elisa Mirotsou, Maria Sablitzky, Fred |
description | The neuron‐specific rat enolase (NSE) promoter was employed to establish transgenic mice expressing Cre recombinase in the central nervous system. Founders were crossed with dormant lacZ indicator mice and specificity as well as efficiency of Cre‐mediated transgene activation was determined by PCR and/or X‐gal staining. Whereas most transgenic lines exhibited Cre activity in early development resulting in widespread Cre activity, one line (NSE‐Cre26) expressed high levels of Cre in the developing and adult brain. With the exception of kidney, which showed occasionally low level of Cre activity, Cre recombination in double transgenics was restricted to the nervous system. Whole‐mount X‐gal staining of 9.5 dpc embryos indicated Cre‐mediated lacZ expression in forebrain, hindbrain, and along the midbrain flexure. A similar expression pattern was observed during later stages of embryogenesis (11.5–13.5 dpc). In adult mice, Cre recombinase was expressed in cerebral cortex and cerebellum and high levels of Cre‐mediated lacZ expression were observed in hippocampus, cortex, and septum. The NSE‐Cre26 transgenic mouse line thus provides a useful tool to specifically overexpress and/or inactivate genes in the developing and adult brain. genesis 31:118–125, 2001. © 2001 Wiley‐Liss, Inc. |
doi_str_mv | 10.1002/gene.10014 |
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Founders were crossed with dormant lacZ indicator mice and specificity as well as efficiency of Cre‐mediated transgene activation was determined by PCR and/or X‐gal staining. Whereas most transgenic lines exhibited Cre activity in early development resulting in widespread Cre activity, one line (NSE‐Cre26) expressed high levels of Cre in the developing and adult brain. With the exception of kidney, which showed occasionally low level of Cre activity, Cre recombination in double transgenics was restricted to the nervous system. Whole‐mount X‐gal staining of 9.5 dpc embryos indicated Cre‐mediated lacZ expression in forebrain, hindbrain, and along the midbrain flexure. A similar expression pattern was observed during later stages of embryogenesis (11.5–13.5 dpc). In adult mice, Cre recombinase was expressed in cerebral cortex and cerebellum and high levels of Cre‐mediated lacZ expression were observed in hippocampus, cortex, and septum. The NSE‐Cre26 transgenic mouse line thus provides a useful tool to specifically overexpress and/or inactivate genes in the developing and adult brain. genesis 31:118–125, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 1526-954X</identifier><identifier>EISSN: 1526-968X</identifier><identifier>DOI: 10.1002/gene.10014</identifier><identifier>PMID: 11747202</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animals ; Brain - embryology ; Brain - enzymology ; DNA Primers - chemistry ; dormant lacZ indicator mice ; Gene Expression Regulation, Developmental - genetics ; Gene Expression Regulation, Enzymologic - genetics ; Gene Targeting ; Genes, Reporter - physiology ; Immunoenzyme Techniques ; Integrases - genetics ; Integrases - metabolism ; Lac Operon - physiology ; loxP sites ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Mice, Transgenic ; neurogenesis ; Neurons - physiology ; Phosphopyruvate Hydratase - genetics ; Polymerase Chain Reaction ; Promoter Regions, Genetic - physiology ; rat enolase promoter ; site-specific recombination ; Transcriptional Activation ; Transgenes - genetics ; Viral Proteins - genetics ; Viral Proteins - metabolism</subject><ispartof>Genesis (New York, N.Y. : 2000), 2001-11, Vol.31 (3), p.118-125</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>Copyright 2001 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3944-5a5fc9684cc6f6bfbe4d990b121f95bc83a81172c1035f4306dda13a3cb7b5993</citedby><cites>FETCH-LOGICAL-c3944-5a5fc9684cc6f6bfbe4d990b121f95bc83a81172c1035f4306dda13a3cb7b5993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11747202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cinato, Elisa</creatorcontrib><creatorcontrib>Mirotsou, Maria</creatorcontrib><creatorcontrib>Sablitzky, Fred</creatorcontrib><title>Cre-mediated transgene activation in the developing and adult mouse brain</title><title>Genesis (New York, N.Y. : 2000)</title><addtitle>Genesis</addtitle><description>The neuron‐specific rat enolase (NSE) promoter was employed to establish transgenic mice expressing Cre recombinase in the central nervous system. Founders were crossed with dormant lacZ indicator mice and specificity as well as efficiency of Cre‐mediated transgene activation was determined by PCR and/or X‐gal staining. Whereas most transgenic lines exhibited Cre activity in early development resulting in widespread Cre activity, one line (NSE‐Cre26) expressed high levels of Cre in the developing and adult brain. With the exception of kidney, which showed occasionally low level of Cre activity, Cre recombination in double transgenics was restricted to the nervous system. Whole‐mount X‐gal staining of 9.5 dpc embryos indicated Cre‐mediated lacZ expression in forebrain, hindbrain, and along the midbrain flexure. A similar expression pattern was observed during later stages of embryogenesis (11.5–13.5 dpc). In adult mice, Cre recombinase was expressed in cerebral cortex and cerebellum and high levels of Cre‐mediated lacZ expression were observed in hippocampus, cortex, and septum. The NSE‐Cre26 transgenic mouse line thus provides a useful tool to specifically overexpress and/or inactivate genes in the developing and adult brain. genesis 31:118–125, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Brain - embryology</subject><subject>Brain - enzymology</subject><subject>DNA Primers - chemistry</subject><subject>dormant lacZ indicator mice</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Gene Expression Regulation, Enzymologic - genetics</subject><subject>Gene Targeting</subject><subject>Genes, Reporter - physiology</subject><subject>Immunoenzyme Techniques</subject><subject>Integrases - genetics</subject><subject>Integrases - metabolism</subject><subject>Lac Operon - physiology</subject><subject>loxP sites</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Mice, Transgenic</subject><subject>neurogenesis</subject><subject>Neurons - physiology</subject><subject>Phosphopyruvate Hydratase - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>rat enolase promoter</subject><subject>site-specific recombination</subject><subject>Transcriptional Activation</subject><subject>Transgenes - genetics</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkEtPwkAUhSdGI4hu_AFmVi5MqvPsY6kEkUhwIUZ2k-n0FkdLizMF5d9bBHSnq3sX3zk5-RA6peSSEsKuplDC-qNiD7WpZGGQhPFkf_dLMWmhI-9fCSEyZuwQtSiNRMQIa6NB10Ewg8zqGjJcO136dR3WprZLXduqxLbE9QvgDJZQVHNbTrEuM6yzRVHjWbXwgFOnbXmMDnJdeDjZ3g56uu2Nu3fB8KE_6F4PA8MTIQKpZW6afcKYMA_TPAWRJQlJKaN5IlMTcx0385ihhMtccBJmmaZcc5NGqUwS3kHnm965q94X4Gs1s95AUegSmjUqYlyGRLJ_QRozIURIG_BiAxpXee8gV3NnZ9qtFCVqbVitlahvww18tm1dpI23X3SrtAHoBviwBaz-qFL93qi3Kw02Getr-PzJaPemwohHUj2P-uqmmfso7seqy78AlK6UqA</recordid><startdate>200111</startdate><enddate>200111</enddate><creator>Cinato, Elisa</creator><creator>Mirotsou, Maria</creator><creator>Sablitzky, Fred</creator><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200111</creationdate><title>Cre-mediated transgene activation in the developing and adult mouse brain</title><author>Cinato, Elisa ; Mirotsou, Maria ; Sablitzky, Fred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3944-5a5fc9684cc6f6bfbe4d990b121f95bc83a81172c1035f4306dda13a3cb7b5993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Brain - embryology</topic><topic>Brain - enzymology</topic><topic>DNA Primers - chemistry</topic><topic>dormant lacZ indicator mice</topic><topic>Gene Expression Regulation, Developmental - genetics</topic><topic>Gene Expression Regulation, Enzymologic - genetics</topic><topic>Gene Targeting</topic><topic>Genes, Reporter - physiology</topic><topic>Immunoenzyme Techniques</topic><topic>Integrases - genetics</topic><topic>Integrases - metabolism</topic><topic>Lac Operon - physiology</topic><topic>loxP sites</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Mice, Transgenic</topic><topic>neurogenesis</topic><topic>Neurons - physiology</topic><topic>Phosphopyruvate Hydratase - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>rat enolase promoter</topic><topic>site-specific recombination</topic><topic>Transcriptional Activation</topic><topic>Transgenes - genetics</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cinato, Elisa</creatorcontrib><creatorcontrib>Mirotsou, Maria</creatorcontrib><creatorcontrib>Sablitzky, Fred</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genesis (New York, N.Y. : 2000)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cinato, Elisa</au><au>Mirotsou, Maria</au><au>Sablitzky, Fred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cre-mediated transgene activation in the developing and adult mouse brain</atitle><jtitle>Genesis (New York, N.Y. : 2000)</jtitle><addtitle>Genesis</addtitle><date>2001-11</date><risdate>2001</risdate><volume>31</volume><issue>3</issue><spage>118</spage><epage>125</epage><pages>118-125</pages><issn>1526-954X</issn><eissn>1526-968X</eissn><abstract>The neuron‐specific rat enolase (NSE) promoter was employed to establish transgenic mice expressing Cre recombinase in the central nervous system. Founders were crossed with dormant lacZ indicator mice and specificity as well as efficiency of Cre‐mediated transgene activation was determined by PCR and/or X‐gal staining. Whereas most transgenic lines exhibited Cre activity in early development resulting in widespread Cre activity, one line (NSE‐Cre26) expressed high levels of Cre in the developing and adult brain. With the exception of kidney, which showed occasionally low level of Cre activity, Cre recombination in double transgenics was restricted to the nervous system. Whole‐mount X‐gal staining of 9.5 dpc embryos indicated Cre‐mediated lacZ expression in forebrain, hindbrain, and along the midbrain flexure. A similar expression pattern was observed during later stages of embryogenesis (11.5–13.5 dpc). In adult mice, Cre recombinase was expressed in cerebral cortex and cerebellum and high levels of Cre‐mediated lacZ expression were observed in hippocampus, cortex, and septum. The NSE‐Cre26 transgenic mouse line thus provides a useful tool to specifically overexpress and/or inactivate genes in the developing and adult brain. genesis 31:118–125, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11747202</pmid><doi>10.1002/gene.10014</doi><tpages>8</tpages></addata></record> |
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subjects | Animals Brain - embryology Brain - enzymology DNA Primers - chemistry dormant lacZ indicator mice Gene Expression Regulation, Developmental - genetics Gene Expression Regulation, Enzymologic - genetics Gene Targeting Genes, Reporter - physiology Immunoenzyme Techniques Integrases - genetics Integrases - metabolism Lac Operon - physiology loxP sites Mice Mice, Inbred C57BL Mice, Inbred CBA Mice, Transgenic neurogenesis Neurons - physiology Phosphopyruvate Hydratase - genetics Polymerase Chain Reaction Promoter Regions, Genetic - physiology rat enolase promoter site-specific recombination Transcriptional Activation Transgenes - genetics Viral Proteins - genetics Viral Proteins - metabolism |
title | Cre-mediated transgene activation in the developing and adult mouse brain |
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