Fabrication of layer-by-layer deposited multilayer films containing DNA and its interaction with methyl green

Multilayer films were fabricated by layer-by-layer electrostatic deposition techniques between poly(diallyldimethylammonium chloride) (PDDA) and calf thymus DNA (CT DNA) on glassy carbon and quartz substrates. Electrochemical impedance spectroscopy (EIS), Fourier transform infrared (FTIR) spectrosco...

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Bibliographic Details
Published in:Biophysical chemistry 2001-12, Vol.94 (1), p.11-22
Main Authors: Luo, Liqiang, Liu, Jianyun, Wang, Zhenxin, Yang, Xiurong, Dong, Shaojun, Wang, Erkang
Format: Article
Language:English
Subjects:
Animals
Calf thymus DNA
Cattle
DNA - chemistry
Layer-by-layer
Methyl green
Methyl Green - chemistry
Spectroscopy
Spectrum Analysis
Static Electricity
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Summary:Multilayer films were fabricated by layer-by-layer electrostatic deposition techniques between poly(diallyldimethylammonium chloride) (PDDA) and calf thymus DNA (CT DNA) on glassy carbon and quartz substrates. Electrochemical impedance spectroscopy (EIS), Fourier transform infrared (FTIR) spectroscopy and UV-vis spectroscopy demonstrated the uniform assembly of PDDA/DNA multilayer films, and X-ray photoelectron spectroscopy confirmed the elemental composition of the films. Moreover, the interaction of DNA in PDDA/DNA films with methyl green was investigated by UV-vis spectroscopy and circular dichroism (CD).
ISSN:0301-4622
1873-4200
DOI:10.1016/S0301-4622(01)00204-6