Sotolone Production by Hairy Root Cultures of Trigonella foenum-graecum in Airlift with Mesh Bioreactors

3-Hydroxy-4,5-dimethyl-2(5H)-furanone (sotolone) and 3-amino-4,5-dimethyl-2(5H)-furanone, the postulated precursor of sotolone, were detected in hairy root cultures of Trigonella foenum-graecum (fenugreek) by GC-MS. The hairy root cultures in both conical flasks and airlift with mesh bioreactors wer...

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Published in:Journal of agricultural and food chemistry 2001-12, Vol.49 (12), p.6012-6019
Main Authors: Peraza-Luna, Fernando, Rodríguez-Mendiola, Martha, Arias-Castro, Carlos, Bessiere, Jean-Marie, Calva-Calva, Graciano
Format: Article
Language:English
Subjects:
Aroma and flavouring agent industries
Biological and medical sciences
Bioreactors
Biotechnology
Carbohydrate Metabolism
Cells, Cultured
Food industries
Food processing
Fundamental and applied biological sciences. Psychology
Furans - isolation & purification
Furans - metabolism
Gas Chromatography-Mass Spectrometry
Hypocotyl - metabolism
Hypocotyl - microbiology
Industrial applications and implications. Economical aspects
Kinetics
Nitrates - metabolism
Phosphates - metabolism
Plant Oils - isolation & purification
Plant Roots - metabolism
Rhizobium - metabolism
Time Factors
Trigonella - metabolism
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Summary:3-Hydroxy-4,5-dimethyl-2(5H)-furanone (sotolone) and 3-amino-4,5-dimethyl-2(5H)-furanone, the postulated precursor of sotolone, were detected in hairy root cultures of Trigonella foenum-graecum (fenugreek) by GC-MS. The hairy root cultures in both conical flasks and airlift with mesh bioreactors were achieved from hypocotyl of seedling by infection with Agrobacterium rhizogenes. In flasks, the mathematical relationship between hairy root growth and conductivity was established and afterward used to evaluate the biomass evolution in bioreactor cultures due to the difficulty of obtaining direct biomass samples from the bioreactor. The GC-MS analyses of ethanolic extracts from hairy roots revealed the presence of two important compounds:  sotolone (1.2% of the volatile fraction) and 3-amino-4,5-dimethyl-2(5H)-furanone (17% of the volatile fraction). These results point out that biotechnological production of sotolone in bioreactors is possible. Additionally, these hairy root cultures offer, for the first time, an excellent biological model to study the biosynthetic pathway of sotolone in fenugreek. Keywords: Sotolone; 3-amino-4,5-dimethyl-2(5H)-furanone; hairy roots; airlift bioreactor; Trigonella foenum-graecum; fenugreek
ISSN:0021-8561
1520-5118
DOI:10.1021/jf010818j