High spatial resolution measurements of organ blood flow in small laboratory animals

1  Division of Pulmonary and Critical Care Medicine and 2  Department of Physiology and Biophysics, University of Washington, Seattle 98195; and 3  Barlow Scientific, Incorporated, and The Evergreen State College, Olympia, Washington 98505 With the use of a newly developed Imaging Cryomicrotome to d...

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Bibliographic Details
Published in:American journal of physiology. Heart and circulatory physiology 2000-11, Vol.279 (5), p.H2043-H2052
Main Authors: Bernard, Susan L, Ewen, Jon R, Barlow, Clyde H, Kelly, Jeff J, McKinney, Steven, Frazer, David A, Glenny, Robb W
Format: Article
Language:English
Subjects:
Animals
Blood Flow Velocity
Fluorescent Dyes - pharmacokinetics
Kidney - blood supply
Kidney - cytology
Lung - blood supply
Lung - cytology
Microspheres
Models, Cardiovascular
Myocardium - metabolism
Poisson Distribution
Rabbits
Rats
Regional Blood Flow - physiology
Swine
Tissue Distribution
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Summary:1  Division of Pulmonary and Critical Care Medicine and 2  Department of Physiology and Biophysics, University of Washington, Seattle 98195; and 3  Barlow Scientific, Incorporated, and The Evergreen State College, Olympia, Washington 98505 With the use of a newly developed Imaging Cryomicrotome to determine the spatial location of fluorescent microspheres in organs, we validate and report our processing algorithms for measuring regional blood flow in small laboratory animals. Microspheres (15-µm diameter) of four different fluorescent colors and one radioactive label were simultaneously injected into the left ventricle of a pig. The heart and kidneys were dissected, and the numbers of fluorescent and radioactive microspheres were determined in 10 randomly selected pieces. All microsphere counts fell well within the 95% expected confidence limits as determined from the radioactive counts. Fluorescent microspheres (15-µm diameter) of four different colors were also injected into the tail vein of a rat and the left ventricle of a rabbit. After correction for Poisson noise, correlation coefficients between the colors were 0.99 ± 0.02 (means ± SD) for the rabbit heart and 0.99 ±   0.02 for the rat lung. Mathematical dissection algorithms, statistics to analyze the spatial data, and methods to visualize blood flow distributions in small animal organs are presented. fluorescent microspheres; organ perfusion; spatial distribution; heterogeneity
ISSN:0363-6135
1522-1539
DOI:10.1152/ajpheart.2000.279.5.h2043