Relation between in vivo and in vitro measurements of skeletal muscle oxidative metabolism

The relationships between in vivo 31P magnetic resonance spectroscopy (MRS) and in vitro markers of oxidative capacity (mitochondrial function) were determined in 27 women with varying levels of physical fitness. Following 90‐s isometric plantar flexion exercises, calf muscle mitochondrial function...

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Bibliographic Details
Published in:Muscle & nerve 2001-12, Vol.24 (12), p.1665-1676
Main Authors: Larson-Meyer, D. Enette, Newcomer, Bradley R., Hunter, Gary R., Joanisse, Denis R., Weinsier, Roland L., Bamman, Marcas M.
Format: Article
Language:English
Subjects:
31P MRS
Adenosine Diphosphate - metabolism
Adenosine Triphosphate - metabolism
Adult
Biological and medical sciences
Biopsy
calf muscle
citochrome c oxidase
Citrate (si)-Synthase - metabolism
citrate synthase
Electron Transport Complex IV - metabolism
Female
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Isometric Contraction - physiology
Magnetic Resonance Imaging
Middle Aged
Mitochondria - metabolism
mitochondrial function
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
oxidative metabolism
Oxygen Consumption - physiology
Phosphocreatine - metabolism
skeletal muscle fiber type
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:The relationships between in vivo 31P magnetic resonance spectroscopy (MRS) and in vitro markers of oxidative capacity (mitochondrial function) were determined in 27 women with varying levels of physical fitness. Following 90‐s isometric plantar flexion exercises, calf muscle mitochondrial function was determined from the phosphocreatine (PCr) recovery time constant, the adenosine diphosphate (ADP) recovery time constant, the rate of change of PCr during the initial 14 s of recovery, and the apparent maximum rate of oxidative adenosine triphosphate (ATP) synthesis (Qmax). Muscle fiber type distribution (I, IIa, IIx), citrate synthase (CS) activity, and cytochrome c oxidase (COX) activity were determined from a biopsy sample of lateral gastrocnemius. MRS markers of mitochondrial function correlated moderately (P < 0.05) with the percentage of type IIa oxidative fibers (r = 0.41 to 0.66) and CS activity (r = 0.48 to 0.64), but only weakly with COX activity (r = 0.03 to 0.26, P > 0.05). These results support the use of MRS to determine mitochondrial function in vivo. © 2001 John Wiley & Sons, Inc. Muscle Nerve 24: 1665–1676, 2001
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.1202