Partitioning of Amino Acids Flowing to the Abomasum into Feed, Bacterial, Protozoal, and Endogenous Fractions

We partitioned the flow of amino acids (AA) to the abomasum among rumen undegradable protein (RUP) and bacterial, protozoal, and endogenous fractions using four Holstein cows in midlactation that were equipped with ruminal and abomasal cannulas. A 2 × 2 factorial design with four diets, combinations...

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Bibliographic Details
Published in:Journal of dairy science 2000-10, Vol.83 (10), p.2326-2334
Main Authors: Shabi, Z., Tagari, H., Murphy, M.R., Bruckental, I., Mabjeesh, S.J., Zamwel, S., Celik, K., Arieli, A.
Format: Article
Language:English
Subjects:
AA profile
Abomasum - metabolism
Amino Acids - analysis
Amino Acids - metabolism
Animal productions
Animals
bacteria
Bacteria - chemistry
Bacteria - isolation & purification
Bacterial Proteins - analysis
Biological and medical sciences
Catheterization
Cattle
Dietary Proteins - analysis
Dietary Proteins - metabolism
Eukaryota - chemistry
Eukaryota - isolation & purification
Female
Fundamental and applied biological sciences. Psychology
General aspects
Lactation
protozoa
Protozoan Proteins - analysis
Rumen - metabolism
Rumen - microbiology
Rumen - parasitology
Terrestrial animal productions
Vertebrates
Vertebrates: digestive system
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Summary:We partitioned the flow of amino acids (AA) to the abomasum among rumen undegradable protein (RUP) and bacterial, protozoal, and endogenous fractions using four Holstein cows in midlactation that were equipped with ruminal and abomasal cannulas. A 2 × 2 factorial design with four diets, combinations of high or low ruminally degradable organic matter, and rumen degradable protein, was employed. Crude protein (CP) and AA contents of ruminal bacteria and protozoa and abomasal digesta were determined. Equations for the source compositions and in vivo flows of CP and 16 AA were then solved simultaneously with a linear program to estimate the contribution of RUP, bacterial, protozoal, and endogenous CP to AA flows. The flows of RUP and bacterial AA were not affected by diet. Low dietary RDP increased the flow of protozoal AA to the abomasum, but the ruminally degradable organic matter content of the diet did not affect protozoal AA flow. Across diets, RUP, bacterial, protozoal, and endogenous fractions provided 55, 33, 11, and
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(00)75120-4