Controlled ion fragmentation in a 2-D quadrupole ion trap for external ion accumulation in ESI FTICR mass spectrometry

Undesired fragmentation of electrospray generated ions in an rf multipole traps can be problematic in many applications. Of special interest here is ion dissociation in a 2-D quadrupole ion trap external to a Fourier transform ion cyclotron resonance mass spectrometer (FTICR MS) used in proteomic st...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2001-12, Vol.12 (12), p.1312-1319
Main Authors: Belov, Mikhail E, Gorshkov, Michael V, Udseth, Harold R, Smith, Richard D
Format: Article
Language:English
Subjects:
Algorithms
Aminoacids, peptides. Hormones. Neuropeptides
Analytical chemistry
Analytical, structural and metabolic biochemistry
Angiotensin I - chemistry
Biological and medical sciences
Bradykinin - chemistry
Chemistry
Cyclotron resonance
Exact sciences and technology
Fourier Analysis
Fourier transforms
Fragmentation
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry - methods
Gramicidin - chemistry
Ions
Liquid chromatography
Mass spectrometry
Parameter identification
Peptides - chemistry
Proteins
Quadrupoles
Spectrometric and optical methods
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Summary:Undesired fragmentation of electrospray generated ions in an rf multipole traps can be problematic in many applications. Of special interest here is ion dissociation in a 2-D quadrupole ion trap external to a Fourier transform ion cyclotron resonance mass spectrometer (FTICR MS) used in proteomic studies. In this work, we identified the experimental parameters that determine the efficiency of ion fragmentation. We have found that under the pressure conditions used in this study there is a specific combination of the radial and axial potential well depths that determines the fragmentation threshold. This combination of rf and dc fields appears to be universal for ions of different mass-to-charge ratios, molecular weights, and charge states. Such universality allows the fragmentation efficiency of the trapped ions in the course of capillary liquid chromatography (LC) separation studied to be controlled and can increase the useful duty cycle and dynamic range of a FTICR mass spectrometer equipped with an external rf only 2-D quadrupole ion trap.
ISSN:1044-0305
1879-1123
DOI:10.1016/S1044-0305(01)00318-X