Role for p300 in Pax 8 Induction of Thyroperoxidase Gene Expression

The nuclear p300 protein functions as a co-activator of gene transcription. Here we show that p300 works as a co-activator of the transcription factor Pax 8 on the thyroperoxidase gene promoter. Consistent with its role as co-activator, p300 potentiates Pax 8-activated transcription. Furthermore, we...

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Bibliographic Details
Published in:The Journal of biological chemistry 2000-11, Vol.275 (44), p.34100-34105
Main Authors: De Leo, Rossana, Miccadei, Stefania, Zammarchi, Enrico, Civitareale, Donato
Format: Article
Language:English
Subjects:
Adenovirus
AE1A protein
Base Sequence
DNA Primers
DNA-Binding Proteins - metabolism
DNA-Binding Proteins - physiology
Gene Expression Regulation, Enzymologic - physiology
HeLa Cells
Humans
Iodide Peroxidase - genetics
Nuclear Proteins - metabolism
Nuclear Proteins - physiology
p300 protein
Paired Box Transcription Factors
Pax8 protein
PAX8 Transcription Factor
Protein Binding
thyroperoxidase
Trans-Activators - metabolism
Trans-Activators - physiology
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Summary:The nuclear p300 protein functions as a co-activator of gene transcription. Here we show that p300 works as a co-activator of the transcription factor Pax 8 on the thyroperoxidase gene promoter. Consistent with its role as co-activator, p300 potentiates Pax 8-activated transcription. Furthermore, we provide evidence supporting the formation of a complex between both factorsin vivo and in vitro. This interaction involves the amino-terminal and CH3 domains of p300 and the trans-activation domain of Pax 8 at its carboxyl-terminal end. We show that the CH3 domain is crucial for the co-activator role of p300 on the thyroperoxidase gene promoter. In agreement with our finding and with the ability of the adenoviral protein E1A to bind p300, we show that E1A down-regulates Pax 8 activity.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M003043200