Loss of heterozygosity at 13q14 and 13q21 in high grade, high stage prostate cancer

• Published in: The Prostate 2001-11, Vol.49 (3), p.166-171
• Main Authors: Dong, Jin-Tang, Boyd, James C., Frierson Jr, Henry F.
• Format: Article
• Language: English
• Subjects: 13q14; 13q21; 13q33; Aged; Aged, 80 and over; Biological and medical sciences; chromosome 13; Chromosomes, Human, Pair 13 - genetics; DNA, Neoplasm - genetics; DNA, Neoplasm - isolation & purification; Electrophoresis, Polyacrylamide Gel; Humans; LOH; Loss of Heterozygosity - genetics; Male; Medical sciences; Microsatellite Repeats; Middle Aged; Neoplasm Staging; Nephrology. Urinary tract diseases; Polymerase Chain Reaction; prostate cancer; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; Tumors of the urinary system; Urinary tract. Prostate gland
• ISSN: 0270-4137; 1097-0045
• DOI: 10.1002/pros.1131

Background Loss of heterozygosity (LOH) at chromosome 13q has been frequently detected in prostate cancer, and three regions (i.e., 13q14, 13q21, and 13q33) may harbor tumor suppressor genes important in this neoplasm. In this study, we examined the frequency of 13q LOH in advanced prostate cancers, in order to determine the clinicopathologic relevance of 13q LOH. Methods LOH was determined by analyzing microsatellite markers in 41 cases of microdissected predominantly high grade prostate cancer tissues and their matched nonneoplastic cells. The results were compared with those generated previously for lower grade, asymptomatic cancers. Results The frequencies of LOH at 13q14, 13q21, and 13q33 were 62% (21/34), 57% (20/35), and 34% (11/32), respectively. In comparison to previous results, LOH at 13q14 and 13q21 but not 13q33 was more frequent in prostate cancers that produced local clinical symptoms (bladder outlet obstruction) than those that did not (P