Involvement of endometrial membrane sulphydryl groups in blastocyst implantation: sulphydryl groups as a potential target for contraceptive research

The role of membrane sulphydryl groups in blastocyst implantation was studied by masking the membrane sulphydryl groups in the endometrium of Swiss albino mice, Mus musculus, using 10(-5) M cobalt chloride and 0.05 mM as well as 0.005 mM n-ethylmaleimide. Here we show that the blocking of sulphydryl...

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Bibliographic Details
Published in:Contraception (Stoneham) 2001-10, Vol.64 (4), p.255-259
Main Authors: NIVSARKAR, Manish, SETHI, Aditya, BAPU, Cherian, PATEL, Manoj, PADH, Harish
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blastocyst - physiology
Cobalt - metabolism
Contraception
Embryo Implantation - physiology
Endometrium - physiology
Female
Fundamental and applied biological sciences. Psychology
Lipid Peroxidation - physiology
Malondialdehyde - analysis
Mammalian reproduction. General aspects
Mice
Pregnancy
Sulfhydryl Compounds - analysis
Sulfhydryl Compounds - metabolism
Superoxide Dismutase - analysis
Superoxide Dismutase - metabolism
Superoxides - metabolism
Thiobarbiturates - analysis
Vertebrates: reproduction
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Summary:The role of membrane sulphydryl groups in blastocyst implantation was studied by masking the membrane sulphydryl groups in the endometrium of Swiss albino mice, Mus musculus, using 10(-5) M cobalt chloride and 0.05 mM as well as 0.005 mM n-ethylmaleimide. Here we show that the blocking of sulphydryl groups with cobalt resulted in a decrease in superoxide radical surge and an increase in superoxide dismutase levels at the time of implantation. We hypothesize that it may be due to either a decrease in membrane fluidity or the unavailability of sulphydryl groups of endometrial membrane, thus preventing blastocyst implantation. These sulphydryl groups can be targeted for future contraceptive research.
ISSN:0010-7824
1879-0518
DOI:10.1016/S0010-7824(01)00254-2