Mitochondrial AAA-Type Protease Yme1p Is Involved in Bax Effects on Cytochrome c Oxidase

Expression of the pro-apoptotic protein Bax in yeast Saccharomyces cerevisiae induces a release of cytochrome c accompanied by a decrease of the amount of cytochrome c oxidase. Here we show that the decrease of cytochrome c oxidase is due to the activation of mitochondrial protease Yme1p, of which c...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-12, Vol.289 (5), p.1314-1319
Main Authors: Manon, Stéphen, Priault, Muriel, Camougrand, Nadine
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
Apoptosis - physiology
ATP-Dependent Proteases
bcl-2-Associated X Protein
Cell Respiration
Electron Transport Complex IV - metabolism
Genes, Fungal
Genes, myc
Humans
In Vitro Techniques
Mitochondria - enzymology
Phenotype
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-bcl-2
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
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Summary:Expression of the pro-apoptotic protein Bax in yeast Saccharomyces cerevisiae induces a release of cytochrome c accompanied by a decrease of the amount of cytochrome c oxidase. Here we show that the decrease of cytochrome c oxidase is due to the activation of mitochondrial protease Yme1p, of which cytochrome c oxidase subunit 2 (Cox2p) is a substrate. The absence of Yme1p slightly delays Bax-induced cell death, suggesting a role of this protease in yeast cell death and thus of its mammalian homologue in apoptosis.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.6120