A COMPARED STUDY ON THE PURIFICATION OF FETAL AND ADULT RAT HEPATOCYTE SUSPENSIONS BY BIOMAGNETIC ISOLATION TECHNIQUES AND FLOW CYTOMETRY ANALYSIS

An analysis of liver cell populations from both adult and 21 day pregnancy rat fetuses (E21) was carried out. The results show that E21 hepatocytes express OX-43, as do endothelial cells but not adult hepatocytes. OX-43 could be used in future as a cell marker for the hepatocyte maturation.

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Bibliographic Details
Published in:Cell biology international 2001-12, Vol.25 (12), p.1285-1289
Main Authors: Arza, E., Cubero, F.J., Garcı́a-Barrutia, M.S., Ortiz, A., Maganto, P., Mula, N., Arahuetes, R.M.
Format: Article
Language:English
Subjects:
albumin
Animals
Antibodies, Monoclonal
Cells, Cultured
Female
Flow Cytometry
hepatocyte
Hepatocytes - cytology
Immunomagnetic Separation
Liver - cytology
Liver - embryology
Male
Microscopy, Confocal
OX-1
OX-43
OX-44
Pregnancy
Rats
Rats, Wistar
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Description
Summary:An analysis of liver cell populations from both adult and 21 day pregnancy rat fetuses (E21) was carried out. The results show that E21 hepatocytes express OX-43, as do endothelial cells but not adult hepatocytes. OX-43 could be used in future as a cell marker for the hepatocyte maturation.
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.2001.0814