Reactive oxygen species generated from the mitochondrial electron transport chain induce cytochrome c dissociation from beef-heart submitochondrial particles via cardiolipin peroxidation. Possible role in the apoptosis

Cytochrome c release from mitochondria is a critical event in the apoptosis induction. Dissociation of cytochrome c from the mitochondrial inner membrane (IMM) is a necessary first step for cytochrome c release. In the present study, the effect of reactive oxygen species (ROS) on the dissociation of...

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Bibliographic Details
Published in:FEBS letters 2001-12, Vol.509 (3), p.435-438
Main Authors: Petrosillo, Giuseppe, Ruggiero, Francesca Maria, Pistolese, Marilva, Paradies, Giuseppe
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Cardiolipin
Cardiolipins - metabolism
Catalase - metabolism
Cattle
Cytochrome c
Cytochrome c Group - metabolism
Electron Transport
Free Radicals - metabolism
Lipid Peroxidation
Mitochondria - metabolism
Myocardium - cytology
Myocardium - metabolism
Oxygen - metabolism
Reactive oxygen species
Reactive Oxygen Species - metabolism
Superoxide Dismutase - metabolism
Time Factors
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Summary:Cytochrome c release from mitochondria is a critical event in the apoptosis induction. Dissociation of cytochrome c from the mitochondrial inner membrane (IMM) is a necessary first step for cytochrome c release. In the present study, the effect of reactive oxygen species (ROS) on the dissociation of cytochrome c from beef-heart submitochondrial particles (SMP) and on the cardiolipin content was investigated. Exposure of SMP to mitochondrial-mediated ROS generation resulted in a large dissociation of cytochrome c from SMP and in a parallel loss of cardiolipin. Both these effects were directly and significantly correlated and also abolished by superoxide dismutase+catalase. These results demonstrate that ROS generation induces the dissociation of cytochrome c from IMM via cardiolipin peroxidation. The data may prove useful in clarifying the molecular mechanism underlying the release of cytochrome c from the mitochondria to the cytosol.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)03206-9