Comparative phylogeny of the ammonia monooxygenase subunit A and 16S rRNA genes of ammonia-oxidizing bacteria

Abstract A fragment of the ammonia monooxygenase gene (amoA) from 31 strains of ammonia-oxidizing bacteria (AOB) was sequenced and analysed phylogenetically. The results were compared with the phylogeny of 16S rDNA from AOB. For most groups of AOB we found a high consistency between the phylogenetic...

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Bibliographic Details
Published in:FEMS microbiology letters 2001-12, Vol.205 (2), p.237-242
Main Authors: Aakra, Ågot, Utåker, Janne B, Nes, Ingolf F
Format: Article
Language:English
Subjects:
16S rRNA gene
Ammonia
Ammonia monooxygenase
Ammonia-oxidizing bacteria
Ammonia‐oxidizing bacterium
amoA gene
Bacteria
Bacteriology
Biological and medical sciences
Comparative phylogeny
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
Microbiology
Microorganisms
Oxidation
Oxidoreductases - classification
Oxidoreductases - genetics
Phylogenetics
Phylogeny
Proteobacteria - classification
Proteobacteria - enzymology
Proteobacteria - genetics
RNA, Bacterial - classification
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - classification
RNA, Ribosomal, 16S - genetics
rRNA 16S
Species Specificity
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:Abstract A fragment of the ammonia monooxygenase gene (amoA) from 31 strains of ammonia-oxidizing bacteria (AOB) was sequenced and analysed phylogenetically. The results were compared with the phylogeny of 16S rDNA from AOB. For most groups of AOB we found a high consistency between the phylogenetic trees based on the 16S rDNA and amoA sequences. Although it is not a phylogenetic marker, using the amoA as a probe when studying microbial diversity will probably reduce the amount of non-AOB detected, compared to using rDNA based probes. The data presented in this paper extend and improve the basis for application of amoA in studies of AOB in the environment.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2001.tb10954.x