Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Bacillus megaterium IWG3

Glucose dehydrogenase from Bacillus megaterium IWG3 has been crystallized in the presence of NAD+ using the hanging‐drop vapour‐diffusion method with PEG 2000 as the precipitant. Crystals belong to space group C2 and have unit‐cell parameters a = 120.8 (1), b = 66.7 (1), c = 119.6 (1) Å, β = 93.25 (...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2000-11, Vol.56 (11), p.1443-1445
Main Authors: Yamamoto, Keizo, Kusunoki, Masami, Urabe, Itaru, Tabata, Shiro, Osaki, Shigeyoshi
Format: Article
Language:English
Subjects:
B. megaterium
Bacillus megaterium - enzymology
Crystallization
Crystallography, X-Ray
Glucose 1-Dehydrogenase
glucose dehydrogenase
Glucose Dehydrogenases - chemistry
oxidoreductases
Protein Conformation
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Summary:Glucose dehydrogenase from Bacillus megaterium IWG3 has been crystallized in the presence of NAD+ using the hanging‐drop vapour‐diffusion method with PEG 2000 as the precipitant. Crystals belong to space group C2 and have unit‐cell parameters a = 120.8 (1), b = 66.7 (1), c = 119.6 (1) Å, β = 93.25 (3)° with standard deviations in parentheses. Assumption of four subunits in the asymmetric unit gave the most probable Matthews coefficient VM of 2.1 Å3 Da−1 (solvent content 41.7% by volume). X‐ray diffraction data were collected to 1.7 Å on a synchrotron‐radiation source.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S090744490000994X