Usefulness of (99m)Tc-d,l-HMPAO for estimation of GSH content in tumor tissues

• Published in: Nuclear medicine and biology 2000-08, Vol.27 (6), p.577-580
• Main Authors: Shimura, N, Musya, A, Hashimoto, T, Kojima, S, Kubodera, A, Sasaki, T
• Format: Article
• Language: English
• Subjects: Animals; Brain - diagnostic imaging; Brain - metabolism; Carcinoma, Ehrlich Tumor - diagnostic imaging; Carcinoma, Ehrlich Tumor - metabolism; Glutathione - metabolism; Kidney - diagnostic imaging; Kidney - metabolism; Liver - diagnostic imaging; Liver - metabolism; Lung - diagnostic imaging; Lung - metabolism; Male; Maleates - pharmacology; Mice; Mice, Inbred Strains; Neoplasm Transplantation; Organ Specificity - drug effects; Radionuclide Imaging; Spleen - diagnostic imaging; Spleen - metabolism; Stereoisomerism; Technetium Tc 99m Exametazime - metabolism; Technetium Tc 99m Exametazime - pharmacokinetics; Tissue Distribution
• ISSN: 0969-8051
• DOI: 10.1016/S0969-8051(00)00131-1

To investigate whether [(99m)Tc]-hexamethyl propyleneamine oxime ([(99m)Tc]-HMPAO) is applicable for evaluating glutathione (GSH) localization in tumor, the difference of distribution between [(99m)Tc]-d,l- and meso-HMPAO was studied using a mouse tumor model. Biodistribution of [(99m)Tc]-d,l- or meso-HMPAO was studied in GSH-depleted and control Ehrlich tumor-bearing mice. GSH levels in tumors in GSH-depleted and control mice were measured in another set of mice. The uptake of [(99m)Tc]-d,l-HMPAO in tumor was significantly decreased by the diethyl maleate (DEM) treatment. On the other hand, the DEM treatment increased the accumulation of [(99m)Tc]-meso-HMPAO in tumor. Meanwhile, the content of GSH was lowest in tumor among the tissues tested and decreased in a manner similar to other tissues on preloading of DEM. [(99m)Tc]-d,l-HMPAO may be useful for estimating the GSH status in a certain tumor and thereby contribute to the diagnosis of anticancer therapy.