Transgenic expression of cecropin B, an antibacterial peptide from Bombyx mori, confers enhanced resistance to bacterial leaf blight in rice

The short persistence of cecropin B peptide in plants, due to post-translational degradation, is a serious impediment in its effective utilization for developing bacterial resistance transgenic plants. Two DNA constructs encoding the full-length precursor of cecropin B peptide and the mature sequenc...

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Bibliographic Details
Published in:FEBS letters 2000-10, Vol.484 (1), p.7-11
Main Authors: Sharma, Arun, Sharma, Rashmi, Imamura, Morikazu, Yamakawa, Minoru, Machii, Hiroaki
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - metabolism
Antibacterial peptide
Bacterial leaf blight (Xanthomonas oryzae pv. oryzae)
Blotting, Western
Bombyx - genetics
Cecropin-transgenic rice
Disease resistance
Disease Susceptibility
Insect Proteins - genetics
Insect Proteins - metabolism
Oryza - genetics
Oryza - metabolism
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Leaves - metabolism
Plants, Genetically Modified
Protein Transport
RNA, Messenger - analysis
RNA, Messenger - genetics
RNA, Plant - analysis
RNA, Plant - genetics
Signal peptide
Transformation, Genetic
Xanthomonas - physiology
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Summary:The short persistence of cecropin B peptide in plants, due to post-translational degradation, is a serious impediment in its effective utilization for developing bacterial resistance transgenic plants. Two DNA constructs encoding the full-length precursor of cecropin B peptide and the mature sequence of cecropin B peptide preceded by a signal peptide derived from rice chitinase gene were transformed in rice. The differences in the transcriptional levels in independent transgenic lines showed moderate to high expression of cecropin B gene that correlated well with the differences in cecropin B accumulation observed by Western blot analysis. The development of lesions resulting from infection by Xanthomonas oryzae pv. oryzae was significantly confined in the infected leaflet of transgenic lines, when compared with the control plants.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(00)02106-2