Column-switching HPLC for the analysis of plasma in PET imaging studies

A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [ 11C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture colu...

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Bibliographic Details
Published in:Nuclear medicine and biology 2000-08, Vol.27 (6), p.627-630
Main Authors: Hilton, John, Yokoi, Fuji, Dannals, Robert F, Ravert, Hayden T, Szabo, Zsolt, Wong, Dean F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Cocaine - analogs & derivatives
Cocaine - blood
Column-switch
Dopamine Agents - blood
HPLC
Imidazoles - blood
Investigative techniques, diagnostic techniques (general aspects)
Isoquinolines - blood
Ligands
Medical sciences
Miscellaneous. Technology
PET
Plasma
Pyridines - blood
Raclopride - blood
Radionuclide investigations
Sensitivity and Specificity
Solvents - chemistry
Tetrabenazine - analogs & derivatives
Tetrabenazine - blood
Time Factors
Tomography, Emission-Computed
Urea - chemistry
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Description
Summary:A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [ 11C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea.
ISSN:0969-8051
1872-9614
DOI:10.1016/S0969-8051(00)00125-6