A Novel G-Protein-Coupled Receptor Gene Expressed in Striatum

Differential display screening for region-specific transcripts in rat brain revealed a novel striatum-specific transcript encoding an orphan G-protein-coupled receptor (GPCR) designated Strg/Gpr88 for striatum-specific GPCR. We isolated its homologues from human (HGMW-approved symbol GPR88) and mous...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2000-11, Vol.69 (3), p.314-321
Main Authors: Mizushima, Kazuyuki, Miyamoto, Yasuhide, Tsukahara, Fujiko, Hirai, Momoki, Sakaki, Yoshiyuki, Ito, Takashi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cell receptors
Cell structures and functions
chromosome 1
chromosome 3
Chromosome Mapping
Chromosomes, Human, Pair 1
Corpus Striatum - metabolism
DNA, Complementary
Fluorescence
Fundamental and applied biological sciences. Psychology
G-protein-coupled receptor
Gene Expression
Gene Expression Profiling - methods
GPR88 gene
Gpr88 protein
GTP-Binding Proteins - metabolism
Humans
Male
Mice
Miscellaneous
Molecular and cellular biology
Molecular Sequence Data
Rats
Rats, Wistar
Receptors, Cell Surface - chemistry
Receptors, Cell Surface - classification
Receptors, Cell Surface - genetics
Receptors, G-Protein-Coupled
Sequence Homology, Amino Acid
Strg protein
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Summary:Differential display screening for region-specific transcripts in rat brain revealed a novel striatum-specific transcript encoding an orphan G-protein-coupled receptor (GPCR) designated Strg/Gpr88 for striatum-specific GPCR. We isolated its homologues from human (HGMW-approved symbol GPR88) and mouse and mapped them to chromosomes 1p21.3 and 3G1, respectively. These loci are syntenic to each other, thereby suggesting their orthology. The predicted primary sequences of Strg/Gpr88 proteins are highly conserved between human and rodents and show the highest level of homology to receptors for biogenic amines. However, Strg/Gpr88 lacks some residues conserved in all known biogenic amine receptors and hence may represent a novel subtype of GPCR. Northern blot and in situ hybridization analyses revealed that Strg/Gpr88 transcripts are expressed almost exclusively in striatum in both human and rodents. Remarkable conservation in primary structure and a unique expression pattern may indicate a role for Strg/Gpr88 in the fundamental functions of striatum such as the control of motor behavior.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.2000.6340