Characterization of fimN, a new Bordetella bronchiseptica major fimbrial subunit gene

• Published in: Gene 2000-10, Vol.256 (1), p.149-155
• Main Authors: Kania, S.A., Rajeev, S., Burns, E.H., Odom, T.F., Holloway, S.M., Bemis, D.A.
• Format: Article
• Language: English
• Subjects: Activator binding region; Adhesion; Amino Acid Sequence; Animals; Antigens, Bacterial - genetics; Antigens, Bacterial - metabolism; Bacterial Adhesion; Base Sequence; Bordetella bronchiseptica; Bordetella bronchiseptica - genetics; C-stretch; Cercopithecus aethiops; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; fim2 gene; Fim2 protein; fim3 gene; Fim3 protein; Fimbriae Proteins; Fimbriae, Bacterial - genetics; Fimbriae, Bacterial - metabolism; fimN gene; FimN protein; fimX gene; Gene Cloning; Molecular Sequence Data; Respiratory; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Vero Cells; Virulence Factors, Bordetella
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(00)00360-7

Fimbrial proteins play an important role in the binding of Bordetella bronchiseptica to mammalian cells, an event that is key to the pathogenesis of this organism. The fimbrial phenotype of B. bronchiseptica isolates is usually defined serologically by Fim2 and Fim3 antigens. In this study, a previously unidentified fimbrial gene, fimN, was cloned and sequenced. The identity of fimN is based on several observations. The predicted FimN protein has 59.4 and 52.2% homology with B. bronchiseptica Fim2 and Fim3, respectively, and is similar in size to these fimbriae. fimN, expressed as a recombinant protein, is recognized by mAb prepared against Fim2 from Bordetella pertussis. The fimN promoter region contains a stretch of cytosine residues similar in length to those of other fimbrial genes expressed by Bordetella species. It also has an activator binding region, upstream from the C-stretch, that closely resembles a corresponding bvg regulated region in fim2, fim3, and fimX. The fimN gene was isolated from a cosmid prepared with B. bronchiseptica genomic DNA that restored normal properties of cellular adhesion to an adhesion deficient strain of B. bronchiseptica. As such, FimN may be a previously overlooked fimbrial antigen and may play an important role in the pathogenicity of B. bronchiseptica.