Expression of Regulator of G-Protein Signaling Protein-2 Gene in the Rat Ovary at the Time of Ovulation

The ovulatory process in mammals begins when an endogenous surge in LH circulates to the ovary and couples with receptors in the plasma membranes of granulosa cells in mature ovarian follicles. This study provides evidence that the ovulatory stimulus includes induction of the gene for regulator of G...

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Bibliographic Details
Published in:Biology of reproduction 2000-11, Vol.63 (5), p.1513-1517
Main Authors: UJIOKA, Takeshi, RUSSELL, Darryl L, OKAMURA, Hitoshi, RICHARDS, Joanne S, ESPEY, Lawrence L
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental - physiology
Granulosa Cells - drug effects
Granulosa Cells - physiology
Hormone metabolism and regulation
In Situ Hybridization
Luteinizing Hormone - biosynthesis
Luteinizing Hormone - genetics
Mammalian female genital system
Molecular Sequence Data
Ovary - metabolism
Ovulation - physiology
Rats
Rats, Wistar
RGS Proteins - biosynthesis
RGS Proteins - genetics
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Vertebrates: reproduction
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Summary:The ovulatory process in mammals begins when an endogenous surge in LH circulates to the ovary and couples with receptors in the plasma membranes of granulosa cells in mature ovarian follicles. This study provides evidence that the ovulatory stimulus includes induction of the gene for regulator of G-protein signaling protein-2 (RGS2). Immature Wistar rats were primed with 10 IU eCG s.c., and 48 h later the 12-h ovulatory process was initiated by 10 IU hCG (a homolog of LH) s.c. Ovarian RNA was extracted at 0, 2, 4, 8, 12, and 24 h after injecting the animals with hCG. The RNA extracts were used for reverse transcription-polymerase chain reaction differential display to detect gene expression in the stimulated ovarian tissue. Two of the amplified cDNAs that were upregulated within 2 h after the ovaries had been stimulated by hCG were homologous to segments of the mouse gene for RGS2. In situ hybridization indicated that the RGS2 mRNA was expressed in the granulosa layer of mature follicles. In conclusion, the gene for RGS2, which is known to regulate membrane signaling pathways, is transcribed in ovarian follicles in response to an ovulatory dose of gonadotropin.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod63.5.1513