Molecular Cloning, Expression, and Chromosomal Mapping of Human Chondroitin 4-Sulfotransferase, Whose Expression Pattern in Human Tissues Is Different from That of Chondroitin 6-Sulfotransferase

Chondroitin 4-sulfotransferase (C4ST) catalyzes the transfer of sulfate from 3′-phospho-adenosine 5′-phosphosulfate to position 4 of the N-acetylgalactosamine residues of chondroitin. We previously reported the cloning of C4ST cDNA from mouse brain. We here report the cloning and expression of human...

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Published in:Journal of biochemistry (Tokyo) 2000-11, Vol.128 (5), p.763-770
Main Authors: Okuda, Tetsuya, Mita, Satoka, Yamauchi, Shinobu, Matsubara, Taeko, Yagi, Fumiko, Yamamori, Daiki, Fukuta, Masakazu, Kuroiwa, Asato, Matsuda, Yoichi, Habuchi, Osami
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Carbohydrate Sulfotransferases
Catalysis
chondroitin sulfate
Chromosome Mapping
Chromosomes, Human, Pair 12
Cloning, Molecular
Gene Expression Regulation, Enzymologic
Humans
Mice
molecular cloning
Molecular Sequence Data
proteoglycan
Rats
sulfotransferase
Sulfotransferases - genetics
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Summary:Chondroitin 4-sulfotransferase (C4ST) catalyzes the transfer of sulfate from 3′-phospho-adenosine 5′-phosphosulfate to position 4 of the N-acetylgalactosamine residues of chondroitin. We previously reported the cloning of C4ST cDNA from mouse brain. We here report the cloning and expression of human C4ST cDNA. The cDNA was isolated from a human fetal brain cDNA library by hybridization with a DNA probe prepared from rat poly (A)+ RNA used for the cloning of mouse C4ST cDNA. The cDNA comprises a single open reading frame that predicts a Type II transmembrane protein composed of 352 amino acids. The protein has an amino acid sequence homology of 96 percnt; with mouse C4ST. When the cDNA was introduced into a eukaryotic expression vector and trans-fected in COS-7 cells, the sulfotransferase activity that transfers sulfate to both chondroitin and desulfated dermatan sulfate was overexpressed. Northern blot analysis indicated that human C4ST mRNAs (6.0 and 1.9 kb) are expressed ubiquitously in various adult human tissues. Dot blot analysis has shown that human C4ST is strongly expressed in colorectal adenocarcinoma and peripheral blood leukocytes, whereas strong expression of human chondroitin 6-sulfotransferase (C6ST) is observed in aorta and testis. These observations suggest that the expression of C4ST and C6ST may be controlled differently in human tissues. The C4ST gene was localized to chromosome 12q23.2-q23.3 by fluorescence in situ hybridization
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a022813