Tandem Mass Spectrometry of Intact Proteins for Characterization of Biomarkers from Bacillus cereus T Spores

Intact protein biomarkers from Bacillus cereus T spores have been analyzed by high-resolution tandem Fourier transform ion cyclotron resonance mass spectrometry. Two techniques have been applied for excitation of the isolated multiply charged precursor ion species: sustained off-resonance irradiatio...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-12, Vol.73 (23), p.5725-5731
Main Authors: DEMIREV, Plamen A., RAMIREZ, Javier, FENSELAU, Catherine
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillus cereus - chemistry
Bacteria
Biological and medical sciences
Biomarkers
Chemistry
Databases, Protein
Fundamental and applied biological sciences. Psychology
Ions
Mass Spectrometry
Molecular and cellular biology
Molecular Sequence Data
Proteins
Spectrum analysis
Spores, Bacterial - chemistry
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Summary:Intact protein biomarkers from Bacillus cereus T spores have been analyzed by high-resolution tandem Fourier transform ion cyclotron resonance mass spectrometry. Two techniques have been applied for excitation of the isolated multiply charged precursor ion species: sustained off-resonance irradiation/collisionally activated dissociation and electron capture dissociation. Fragmentation-derived sequence tags and BLAST sequence similarity proteome database searches allow unequivocal identification of the major biomarker protein with unprecedented specificity. Sequence-specific fragmentation patterns further confirm protein identification. Moreover, methodology combining accurate mass measurements of intact proteins with additional information contained in a proteome database permits tentative assignment of several other protein biomarkers isolated from the B. cereus T spores. We argue that approaches involving tandem MS of protein biomarkers, combined with bioinformatics, can drastically improve the specificity of individual microorganism identification, particularly in complex environments.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac010672n