Leptin activates Stat3, Stat1 and AP-1 in mouse adipose tissue

Intraperitoneal leptin administration to wild-type and ob/ ob mice caused a prompt activation of Stat1 and Stat3, the former to a lesser extent, in epididymal adipose tissue. Immunoblot experiments showed that tyrosine phosphorylation of Stat3 increased in total cellular extracts and that the phosph...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2000-10, Vol.168 (1), p.11-20
Main Authors: Bendinelli, P, Maroni, P, Pecori Giraldi, F, Piccoletti, R
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adipose Tissue - metabolism
Animals
AP-1
Cell Extracts
Cell Nucleus - genetics
Cell Nucleus - metabolism
DNA - metabolism
DNA-Binding Proteins - immunology
DNA-Binding Proteins - metabolism
Immunoblotting
Leptin
Leptin - metabolism
Leptin - pharmacology
Male
Mice
Mice, Inbred C57BL
Mice, Obese
Mouse white adipose tissue
Phosphorylation
Response Elements
Signal Transduction
STAT
STAT1 Transcription Factor
STAT3 Transcription Factor
Trans-Activators - immunology
Trans-Activators - metabolism
Transcription Factor AP-1 - metabolism
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Summary:Intraperitoneal leptin administration to wild-type and ob/ ob mice caused a prompt activation of Stat1 and Stat3, the former to a lesser extent, in epididymal adipose tissue. Immunoblot experiments showed that tyrosine phosphorylation of Stat3 increased in total cellular extracts and that the phosphorylated protein translocated into the nucleus upon leptin treatment. Tyrosine phosphorylation and nuclear translocation of Stat1 were evident only in ob/ ob mice. Gel shift and supershift analyses showed that leptin activated sis-inducible element (SIE) binding activity of adipose nuclear extracts, with Stat3 homodimer as the predominant complex. Stat1/3 heterodimers and Stat1 homodimers take part as well in the response in wild-type and ob/ ob mice, although to a lesser degree. AP-1 binding activity was also induced in adipose tissue by in vivo leptin treatment with a time course that suggests a post-transcriptional inductive mechanism. This effect was greater in the ob/ ob than in wild-type mice. Our data indicate that leptin operates in vivo directly on adipose tissue by triggering responses that modulate gene expression.
ISSN:0303-7207
1872-8057
DOI:10.1016/S0303-7207(00)00313-0