Fas Ligand Costimulates the In Vivo Proliferation of CD8+ T Cells

Fas ligand (FasL/CD95L/APO-1L) is one of a growing number of TNF family members whose triggering costimulates maximal proliferation of activated T cells. In this study we show that maximal Ag-dependent accumulation of transferred TCR-transgenic CD8(+) T cells requires Fas (CD95/APO-1) expression by...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 2000-11, Vol.165 (10), p.5537-5543
Main Authors: Suzuki, Ivy, Martin, Stefan, Boursalian, Tamar E, Beers, Courtney, Fink, Pamela J
Format: Article
Language:English
Subjects:
Adoptive Transfer
Animals
Apoptosis - genetics
Apoptosis - immunology
CD8-Positive T-Lymphocytes - cytology
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - metabolism
CD8-Positive T-Lymphocytes - transplantation
Cell Size - immunology
Clone Cells
Cytotoxicity, Immunologic - genetics
Epitopes, T-Lymphocyte - metabolism
Fas Ligand Protein
fas Receptor - metabolism
Injections, Intravenous
Ligands
Lymphocyte Activation - genetics
Lymphocyte Activation - immunology
Lymphocyte Count
Lymphocyte Transfusion
Membrane Glycoproteins - administration & dosage
Membrane Glycoproteins - biosynthesis
Membrane Glycoproteins - physiology
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred MRL lpr
Mice, Transgenic
Species Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fas ligand (FasL/CD95L/APO-1L) is one of a growing number of TNF family members whose triggering costimulates maximal proliferation of activated T cells. In this study we show that maximal Ag-dependent accumulation of transferred TCR-transgenic CD8(+) T cells requires Fas (CD95/APO-1) expression by the adoptive hosts. Additionally, adoptively transferred FasL(+) CD8(+) T cells demonstrate a 2-fold advantage in Ag-driven expansion over their FasL(-)counterparts. This study illustrates the in vivo role of TCR-dependent FasL costimulation in the Ag-specific proliferation of both heterogeneous and homogeneous populations of primary CD8(+) T cells and long-term CTL lines. Thus, cross-linking FasL on naive and Ag-experienced CD8(+) T cells whose Ag-specific TCRs are engaged is required to drive maximal cellular proliferation in vivo.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.165.10.5537