Sucrose has no beneficial effects on wound healing in rats

• Published in: The European journal of surgery 2000-10, Vol.166 (10), p.818-822
• Main Authors: Kössi, Jyrki A. O., Ekfors, Tauno O., Aaltonen, Vesa, Laato, Matti
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Collagen - drug effects; Degeneration. Regeneration. Wound healing. Graft; Dermatologic Surgical Procedures; Disease Models, Animal; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; Granulation Tissue - drug effects; Male; Medical sciences; Rats; Rats, Sprague-Dawley; Skin - drug effects; Skin plastic surgery; Sucrose - administration & dosage; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Treatment Failure; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Wound Healing - drug effects; Wound Healing - physiology
• ISSN: 1102-4151; 1741-9271
• DOI: 10.1080/110241500447489

Objective: To evaluate the effects of sucrose treatment on the formation of granulation tissue in a standard wound model. Design: Animal study. Setting: University hospital, Finland. Animals: 32 male Sprague‐Dawley rats divided into 4 groups. Interventions: Implantation of viscose cellulose sponge subcutaneously, and daily injection of three concentrations of sucrose (0.01, 0.1 or 1 M) or vehicle for 7 days. Main outcome measures: The amount of granulation tissue measured by chemical analysis and histology. The amount and distribution of types I and III collagen assayed by immunofluorescence. Results: None of the three concentrations altered the amounts of DNA, RNA, hydroxyproline, nitrogen, hexosamines, and uronic acids in granulation tissue. Neither improvement nor deterioration was seen in the growth of granulation tissue in histological specimens. The amount and distribution of types I and III collagen was similar in controls and sucrose‐treated rats. Type III collagen was most abundant near newly‐formed vessels. Neither sucrose nor fructose was found in wound fluid while the concentration of glucose was significantly lower in all test groups than in controls. Conclusions: Sucrose solution had neither beneficial nor deleterious effects on the amount of developing granulation tissue in an experimental wound model. The amount and distribution of types I and III collagens were also not altered by sucrose treatment. Copyright © 2000 Taylor and Francis Ltd.