Expression of Prolactin Gene in Spontaneous Mammary Tumors in Aging Fischer 344 Rats

Expression of extra pituitary prolactin (PRL) has been recently reported in the mammary gland. However, spontaneous mammary tumors occurring in aging rats have not been investigated for PRL production. The present study was undertaken to examine the expression of PRL gene in rat mammary tumors spont...

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Bibliographic Details
Published in:ENDOCRINE JOURNAL 2001, Vol.48(5), pp.597-602
Main Authors: UMEMURA, SHINOBU, IWASAKA, TOSHIKI, KAKIMOTO, KATSUHIRO, TAKAHASHI, AKEMI, KOIZUMI, HARUKO, MIYAKAWA, YOSHIFUMI, KUROTANI, REIKO, OSAMURA, R. YOSHIYUKI
Format: Article
Language:English
Subjects:
Adenocarcinoma
Adenocarcinoma, Bronchiolo-Alveolar - genetics
Adenocarcinoma, Bronchiolo-Alveolar - metabolism
Adenocarcinoma, Bronchiolo-Alveolar - pathology
Animals
Female
Fibroadenoma
Fibroadenoma - genetics
Fibroadenoma - metabolism
Fibroadenoma - pathology
Gene Expression Regulation, Neoplastic
Immunohistochemistry
In Situ Hybridization
Mammary Neoplasms, Animal - genetics
Mammary Neoplasms, Animal - metabolism
Mammary Neoplasms, Animal - pathology
Pituitary Neoplasms - genetics
Pituitary Neoplasms - metabolism
Prolactin
Prolactin - analysis
Prolactin - biosynthesis
Prolactin - genetics
Prolactinoma - genetics
Prolactinoma - metabolism
Rat mammary gland
Rats
Rats, Inbred F344
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Spontaneous tumor
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Summary:Expression of extra pituitary prolactin (PRL) has been recently reported in the mammary gland. However, spontaneous mammary tumors occurring in aging rats have not been investigated for PRL production. The present study was undertaken to examine the expression of PRL gene in rat mammary tumors spontaneously arisen in rats with pituitary prolactinomas among 130 female Fischer-344 (F-344) rats. The tumors examined were fibroadenoma (adenomatous type) in the 18-month old rat and adenocarcinoma (alveolar/tubular type) in the 21-month old rat. PRL mRNA was examined by solution and in situ reverse transcription-polymerase chain reaction (RT-PCR) method. The predicted amplified products for PRL mRNA were identified in both tumors, and its expression was confirmed to be in the cytoplasm of epithelial cells. The results of the present study showed that PRL gene is expressed in spontaneously arising mammary tumors.
ISSN:0918-8959
1348-4540
DOI:10.1507/endocrj.48.597