Increased density of lymphocytes bearing γ/δ T-cell receptors in recurrent aphthous ulceration (RAU)

Lymphocytes bearing the T-cell receptor (TCR) γ/δ are increased in the peripheral blood of patients with recurrent aphthous ulcers (RAU) and Behçet's disease. In this study, we examined whether the density of TCR- γ/δ bearing lymphocytes was also increased locally in RAU lesions. Ten RAU lesion...

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Bibliographic Details
Published in:International journal of oral and maxillofacial surgery 2000-10, Vol.29 (5), p.375-380
Main Authors: Natah, Sirajedin S., Häyrinen-Immonen, Ritva, Hietanen, Jarkko, Patinen, Pertti, Malmström, Maria, Savilahti, Erkki, Konttinen, Yrjö T.
Format: Article
Language:English
Subjects:
Adult
Antibodies, Monoclonal
aphthous ulcers
Biological and medical sciences
Biopsy
Dentistry
Female
Humans
Immunohistochemical methods
Immunohistochemistry
Immunological methods for diagnosis and exploration
Immunopathology
Lymphocyte Count
Male
Medical sciences
Middle Aged
Mouth Mucosa - immunology
Mouth Mucosa - pathology
Phenotype
Receptors, Antigen, T-Cell, gamma-delta - immunology
Recurrence
Reference Values
Serological tests
Stomatitis, Aphthous - immunology
Stomatitis, Aphthous - pathology
T-cell receptors
T-Lymphocytes - immunology
γ/δ T-cells
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Summary:Lymphocytes bearing the T-cell receptor (TCR) γ/δ are increased in the peripheral blood of patients with recurrent aphthous ulcers (RAU) and Behçet's disease. In this study, we examined whether the density of TCR- γ/δ bearing lymphocytes was also increased locally in RAU lesions. Ten RAU lesions from ten patients were compared with ulcer-free mucosa from sites contralateral to the lesions, and with 10 samples of clinically healthy oral mucosa taken from 10 healthy volunteers. Samples were labeled with a panel of monoclonal antibodies specific to CD3, α/β TCR and γ/δ TCR in avidin-biotin-peroxidase complex (ABC) staining. Lymphocytes expressing γ/δ TCRs were very low in non-lesional mucosa and clinically healthy mucosa. By contrast, γ/δ T-cells were numerous and observed in all RAU lesions especially within the epithelium, inflammatory infiltrates and at perivascular locations. The count of γ/δ T-cells was high in connective tissue of RAU (200±126 cells/mm 2) compared with connective tissue of controls (4±4 cells/mm 2; P
ISSN:0901-5027
1399-0020
DOI:10.1016/S0901-5027(00)80057-0